Kumar R, Singh S K, Koshkin A A, Rajwanshi V K, Meldgaard M, Wengel J
Department of Chemistry, University of Copenhagen, Denmark.
Bioorg Med Chem Lett. 1998 Aug 18;8(16):2219-22. doi: 10.1016/s0960-894x(98)00366-7.
LNA (Locked Nucleic Acids, 1, X = O, Y = O) is a novel oligonucleotide analogue capable of recognizing complementary DNA and RNA with unprecedented thermal affinities. Synthesis of the first chemically modified LNA analogues is reported. A 9-mer phosphorothioate-LNA containing three LNA thymine monomers (1, X = O, Y = S, Base = thymin-1-yl) and 9-mer LNAs containing one, three or five 2'-thio-LNA monomers (1, X = S, Y = O, Base = uracil-1-yl) were able to recognize both complementary DNA and RNA with thermal affinities comparable to those of parent LNA.
锁核酸（LNA，1，X = O，Y = O）是一种新型寡核苷酸类似物，能够以前所未有的热亲和力识别互补DNA和RNA。本文报道了首个化学修饰的LNA类似物的合成。一种含有三个LNA胸腺嘧啶单体的9聚体硫代磷酸酯-LNA（1，X = O，Y = S，碱基=胸腺嘧啶-1-基）以及含有一个、三个或五个2'-硫代-LNA单体的9聚体LNA（1，X = S，Y = O，碱基=尿嘧啶-1-基）能够以与母体LNA相当的热亲和力识别互补DNA和RNA。
