Gongora R, Figueroa F, Klein J
Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany.
Scand J Immunol. 1998 Dec;48(6):651-8. doi: 10.1046/j.1365-3083.1998.00457.x.
As part of an ongoing project aimed at the characterization of the MHC system in bony fishes, we have attempted to identify the class III region in the zebrafish (a teleost), a region that in higher vertebrates contains genes coding for complement proteins C2, Bf and C4. We obtained several genomic PAC clones by hybridization with a zebrafish Bf probe, previously identified in our laboratory, and searched these for the presence of other class III genes. We were able to obtain a second Bf-like gene, however, we were unable to detect any C2- or C4-like genes. By using highly degenerated primers, we extended our search to a hepatopancreas cDNA library and amplified from it clones corresponding to three different C3-like genes, and also the Bf genes, but not any C2- or C4-like genes. The zebrafish therefore contains two Bf and three C3 loci but apparently no C2 and C4 loci. Independent duplications of the Bf and C3 genes in bony fishes suggest that complement plays a prominent part in the immune response of this class of vertebrates.
作为一个旨在鉴定硬骨鱼类主要组织相容性复合体（MHC）系统的正在进行的项目的一部分，我们试图在斑马鱼（一种硬骨鱼）中鉴定Ⅲ类区域，在高等脊椎动物中该区域包含编码补体蛋白C2、Bf和C4的基因。我们通过与先前在我们实验室中鉴定的斑马鱼Bf探针杂交获得了几个基因组P1人工染色体（PAC）克隆，并在这些克隆中寻找其他Ⅲ类基因的存在。我们能够获得第二个类似Bf的基因，然而，我们未能检测到任何类似C2或C4的基因。通过使用高度简并引物，我们将搜索范围扩展到肝胰腺cDNA文库，并从其中扩增出对应于三个不同类似C3基因以及Bf基因的克隆，但没有类似C2或C4的基因。因此，斑马鱼包含两个Bf和三个C3基因座，但显然没有C2和C4基因座。硬骨鱼类中Bf和C3基因的独立复制表明补体在这类脊椎动物的免疫反应中起重要作用。
