Lee Jang-Wook, Lee Young Mee, Lee Jeong-Ho, Noh Jae Koo, Kim Hyun Chul, Park Choul-Ji, Park Jong-Won, Hwang In Joon, Kim Sung Yeon
Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
Dev Reprod. 2013 Dec;17(4):311-9. doi: 10.12717/DR.2013.17.4.311.
Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs, therefore effective innate mechanisms is very important for survival in such an environment. The key component of innate immune system, C3 is central protein of all activation pathways of the complement system, leading to inflammatory reactions, such as opsonisation, chemotaxis, and cell lysis of pathogens. Although, innate mechanisms is essential for survival in the early stage of development, little is known about defence mechanisms. In this study, the alignment analysis showed that amino acid sequence of C3 from olive flounder liver EST homologous to other known C3 sequences with 73-99% identity. Also, we examined the tissue distribution of olive flounder C3 and analyzed expression pattern from the fertilized egg until 28 days post hatching. As a result, olive flounder C3 mRNA was expressed only in the liver and the mRNA level more increased as developmental proceed during the early stage. These results may suggest that olive flounder C3 plays an important function in the early immune response of olive flounder larvae.
鱼类幼体从孵化到淋巴器官成熟都直接暴露于微生物环境中，因此有效的先天免疫机制对于在这种环境中生存非常重要。先天免疫系统的关键成分C3是补体系统所有激活途径的核心蛋白，可引发炎症反应，如病原体的调理作用、趋化作用和细胞裂解。虽然先天免疫机制对于鱼类幼体早期发育的存活至关重要，但我们对其防御机制了解甚少。在本研究中，序列比对分析表明，牙鲆肝脏EST中的C3氨基酸序列与其他已知C3序列具有73%-99%的同源性。此外，我们检测了牙鲆C3的组织分布，并分析了从受精卵到孵化后28天的表达模式。结果显示，牙鲆C3 mRNA仅在肝脏中表达，并且在早期发育过程中，随着发育进程的推进，mRNA水平进一步升高。这些结果表明，牙鲆C3在牙鲆幼体的早期免疫反应中发挥重要作用。
