Marziali A, Willis T D, Davis R W
Stanford DNA Sequencing and Technology Center, 855 California Avenue, Palo Alto, CA 94304, USA.
Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):61-6. doi: 10.1073/pnas.96.1.61.
A compact, flow-through centrifugation system has been developed specifically for high-throughput centrifugation of large numbers of samples. The instrument is based on multiple high-speed rotors that also serve as sample holders. The small size of the rotors allows them to be arrayed in a standard 96-well microtiter plate spacing, making this device ideal for highly parallel automated instrumentation. Though initially designed for cell separation in DNA sequencing protocols, the flow-through centrifuge can be used to replace conventional centrifugation in most processes involving small samples. Techniques for recovery of both the supernatant and the pellet have been developed, as well as techniques for sample mixing, and cleaning of the reusable rotors. This paper discusses the design and performance of the flow-through centrifuge applied to cell separation and resuspension and to DNA purification and concentration.
一种紧凑的、流通式离心系统已专门开发用于对大量样品进行高通量离心。该仪器基于多个高速转子，这些转子同时也用作样品架。转子体积小，能够以标准96孔微量滴定板间距排列，使得该设备非常适合高度并行的自动化仪器。尽管最初设计用于DNA测序方案中的细胞分离，但在大多数涉及小样品的过程中，流通式离心机可用于替代传统离心。已经开发了回收上清液和沉淀的技术，以及样品混合和清洁可重复使用转子的技术。本文讨论了应用于细胞分离和重悬以及DNA纯化和浓缩的流通式离心机的设计和性能。
