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醋酸纤维素膜免疫电扩散法用于人体疟疾血清学检测(作者译)

[Immuno-electro-diffusion on cellulose acetate membrane for serological testing in human malaria (author's transl)].

作者信息

Druilhe P, Monjour L, Richard-Lenoble D, Gentilini M

出版信息

Pathol Biol (Paris). 1978 Mar-Apr;26(3-4):169-72.

PMID:98748
Abstract

An immuno-electro-diffusion assay for human malaria, using cellulose acetate gel, is described. More than 3500 sera have been tested by this method, which proved very useful for epidemiology since 400 sera a day can be tested by one person, with simple equipment. Its sensitivity compared well to the IFA test, although the antibodies revealed are quite different. A constnat precipitin line appeared, against 56 degrees C labile antigens, in sera of subjects infected by P. falciparum, and up to 8 other precipitins per serum tested. The test may be useful in analyzing these multiple antibodies occurring in patients with malaria.

摘要

本文描述了一种使用醋酸纤维素凝胶的人疟疾免疫电扩散测定法。已有超过3500份血清通过该方法进行检测,该方法对流行病学非常有用,因为一个人每天使用简单设备就能检测400份血清。尽管所检测出的抗体有很大差异,但其灵敏度与免疫荧光抗体试验相当。在感染恶性疟原虫的受试者血清中,针对56℃不稳定抗原出现了一条恒定的沉淀线,每份检测血清中最多还有8条其他沉淀线。该试验可能有助于分析疟疾患者体内出现的这些多种抗体。

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