Luo M, Bilodeau P, Koltunow A, Dennis E S, Peacock W J, Chaudhury A M
Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia.
Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):296-301. doi: 10.1073/pnas.96.1.296.
We have cloned two genes, FIS1 and FIS2, that control both fertilization independent seed development and postpollination embryo development in Arabidopsis. These genes confer female gametophytic phenotypes. FIS2 encodes a protein with a C2H2 zinc-finger motif and three putative nuclear localization signals, indicating that it is likely to be a transcription factor. FIS1 encodes a protein with homology to the Drosophila Polycomb group gene Enhancer-of-zeste and is identical to the recently described Arabidopsis gene MEDEA. FIS1 is a protein with a number of putative functional domains, including the SET domain present in Enhancer-of-zeste-related proteins. Comparison of the position of the lesions in the fis1 and medea mutant alleles indicates that fis1 is a null allele producing a truncated polypeptide lacking all the protein domains whereas the deduced protein from medea lacks only the SET domain. We present a model of the role of FIS1 and FIS2 gene products in seed development.
我们克隆了两个基因，FIS1和FIS2，它们控制拟南芥中不依赖受精的种子发育以及授粉后胚胎发育。这些基因赋予雌配子体表型。FIS2编码一种具有C2H2锌指基序和三个假定核定位信号的蛋白质，这表明它可能是一种转录因子。FIS1编码一种与果蝇多梳基因增强子zeste具有同源性的蛋白质，并且与最近描述的拟南芥基因MEDEA相同。FIS1是一种具有多个假定功能域的蛋白质，包括存在于增强子zeste相关蛋白中的SET结构域。对fis1和medea突变等位基因中损伤位置的比较表明，fis1是一个无效等位基因，产生一种缺少所有蛋白质结构域的截短多肽，而medea推导的蛋白质仅缺少SET结构域。我们提出了FIS1和FIS2基因产物在种子发育中作用的模型。
