Lu B C, Chiu S M
Mol Gen Genet. 1976 Aug 19;147(2):121-7. doi: 10.1007/BF00267563.
Repair synthesis of DNA per se at pachytene is not needed for commitment to meiotic recombination although it is a necessary event to follow. Recombination frequency is governed by the rate of nicking and the time in which unrepaired nicks are allowed to match and crossover. Cold treatment at pachytene prevented repair synthesis, hence open nicks were accumulated to match and crossover, and a 3-fold increase in recombination resulted. The kinetics of cold temperature effect followed a quadratic function as shown by a computer simulation which agreed with our experimental data (Lu, 1974b). High temperature did not change the rate of repair synthesis. It did cause an increased nicking which led to a twofold increase in recombination and which entailed a higher rate of recovery repair synthesis.
尽管减数分裂重组过程中后续需要DNA修复合成这一必要事件，但在粗线期DNA本身的修复合成对于启动减数分裂重组并非必需。重组频率由切口形成速率以及未修复切口进行配对和交叉的时间所决定。粗线期的冷处理阻止了修复合成，因此未封闭的切口得以积累并进行配对和交叉，从而导致重组增加了3倍。如计算机模拟所示，低温效应的动力学遵循二次函数，这与我们的实验数据相符（Lu，1974b）。高温并未改变修复合成的速率。它确实导致切口形成增加，从而使重组增加了两倍，并且需要更高的恢复修复合成速率。
