Tsujimoto H, Hagiwara A, Shimotsuma M, Sakakura C, Osaki K, Sasaki S, Ohyama T, Ohgaki M, Imanishi T, Yamazaki J, Takahashi T
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
J Cancer Res Clin Oncol. 1996;122(10):590-5. doi: 10.1007/BF01221190.
We investigated the significance of milky spots for malignant cells in peritoneal dissemination using three mouse carcinomatous peritonitis models. P388 leukemia and Colon 26 cancer cells were labeled with bromodeoxyuridine (BrdU) and mice were inoculated intraperitoneally. After 24 h the greater omentum and the mesenterium were removed and stained immunohistochemically with anti-BrdU antibody. The labeled cells were found to have preferentially infiltrated into the milky spots in these specimens. Next, using B16 PC melanoma cells, which can be easily distinguished from the other cells by the intrinsic black melanin, the distribution of the melanoma cells was observed macro- and microscopically following intraperitoneal inoculation. The melanoma cells were similarly found to have selectively infiltrated into the milky spots in the omentum and mesenterium after 1 day. Moreover, the melanoma cells were growing and forming distinct metastic lesions within the milky spots 1 week later.
我们使用三种小鼠癌性腹膜炎模型，研究了乳斑对腹膜播散中恶性细胞的意义。用溴脱氧尿苷（BrdU）标记P388白血病细胞和结肠26癌细胞，并将小鼠腹腔内接种。24小时后，切除大网膜和肠系膜，并用抗BrdU抗体进行免疫组织化学染色。发现标记细胞优先浸润到这些标本的乳斑中。接下来，使用B16 PC黑色素瘤细胞，其可通过内在的黑色黑色素与其他细胞轻松区分，腹腔内接种后，宏观和微观观察黑色素瘤细胞的分布。同样发现，1天后黑色素瘤细胞选择性地浸润到网膜和肠系膜的乳斑中。此外，1周后黑色素瘤细胞在乳斑内生长并形成明显的转移灶。
