Shimotsuma M, Simpson-Morgan M W, Takahashi T, Hagiwara A
Developmental Physiology Group, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Cancer Res. 1992 Oct 1;52(19):5400-2.
Omental milky spots are omentum-associated lymphoid tissues that cede peritoneal macrophages and participate in the immunity of the peritoneal cavity. We studied the changing surface features of milky spots and milky spot macrophages of Wistar rats, following the i.p. administration of OK-432, a killed streptococcal preparation (1 Klinische Einheit (unit) in 5 ml of phosphate-buffered saline) by the use of scanning electron microscopy. OK-432-activated macrophages demonstrated marked surface membrane activity and migrated through the stomata of the milky spot into the peritoneal cavity. The characteristic features of activated milky spots and milky spot macrophages were noted as early as 3 h following the administration of OK-432, and continued to be observed until 7 days after the injection. By 14 days after the injection, the structural integrity of the milky spot was partially lost. The activation of milky spots and milky spot macrophages by OK-432 provides a convenient in vivo system for the monitoring and study of i.p. cellular events.
网膜乳斑是与网膜相关的淋巴组织，可产生腹膜巨噬细胞并参与腹腔免疫。我们通过扫描电子显微镜研究了Wistar大鼠腹腔注射OK-432（一种灭活的链球菌制剂，5毫升磷酸盐缓冲盐水中含1临床单位）后乳斑和乳斑巨噬细胞表面特征的变化。OK-432激活的巨噬细胞表现出明显的表面膜活性，并通过乳斑的气孔迁移到腹腔。早在注射OK-432后3小时就注意到了活化乳斑和乳斑巨噬细胞的特征，并持续观察到注射后7天。注射后14天，乳斑的结构完整性部分丧失。OK-432对乳斑和乳斑巨噬细胞的激活为监测和研究腹腔内细胞事件提供了一个方便的体内系统。
