Gilliano N Ia, Bol'shakova O I, Lavrova G A, Konevega L V, Bikineeva E G, Noskin L A
Petersburg Nuclear Physics Institute, Russian Academy of Science, Gatchina.
Radiats Biol Radioecol. 1998 Sep-Oct;38(5):663-71.
It has been studied the correlation of the mitotic activity of the chromosome aberrations and apoptosis, in the V-79 cells pre-exposure to an adapting dose of ionizing radiation from 14C-thymidine prior to an acute challenge dose of gamma-rays. In spite of that the incubation of the cells with isotope increased of the yield of the chromosome aberrations, but the cells became more resistant to following gamma-irradiation. Increasing the adaptive dose of the 14C on degree didn't influence on the present of the adaptive response. However, using concentrations of the 14C damaged metaphase/anaphase transition and cells blocked in this check-point by apoptotic death. The results suggest, that the cellular selection has been involved in 14C-induced adaptive response, estimated by level of asymmetric chromosome aberrations in V-79 cells.
研究了在V - 79细胞中，于急性γ射线照射剂量之前预先暴露于适应剂量的来自14C - 胸腺嘧啶核苷的电离辐射下，染色体畸变的有丝分裂活性与细胞凋亡之间的相关性。尽管用同位素孵育细胞会增加染色体畸变的发生率，但细胞对随后的γ射线照射变得更具抗性。14C适应剂量在一定程度上的增加并不影响适应性反应的出现。然而，使用14C的浓度会破坏中期/后期转换，并且细胞因凋亡死亡而阻滞在这个检查点。结果表明，通过V - 79细胞中不对称染色体畸变水平估计，细胞选择参与了14C诱导的适应性反应。
