Stram Y, Shchori D, Chinitch Y, David D, Molad T, Samina I
Virology Division, Kimron Veterinary Institute, Beit-Dagan, Israel.
Avian Dis. 1998 Oct-Dec;42(4):746-51.
Detection of Newcastle disease virus (NDV) and avian pathotyping of NDV isolates are extremely important because the appearance of virulent virus has significant economic consequences in terms of vaccination, eradication, and the ability to export poultry products. By using nucleotide and amino acid (aa) homology analysis, we could demonstrate that a NDV broiler isolate is a velogenic virus. This analysis was done after mean death time and intracerebral pathogenicity index tests gave inconsistent results. By establishing a nucleotide sequence dendrogram, we found that the disputed Ber-Tuvia was clearly in the same group as the known Herev-Laet, a velogenic isolate. The difference between Ber-Tuvia 92 and the Herev-Laet velogenic isolate was 6% as opposed to > 16% of the meso- and lentogenic isolates. The Ber-Tuvia isolate contains the Arg/Arg and Lys/Arg aa at positions 112, 113 and 115, 116, respectively, in the fusion protein cleavage aa sequence, which is typical for virulent NDV isolates.
新城疫病毒（NDV）的检测以及NDV分离株的禽源分型极其重要，因为强毒病毒的出现会在疫苗接种、根除以及家禽产品出口能力方面造成重大经济后果。通过核苷酸和氨基酸（aa）同源性分析，我们能够证明一株NDV肉鸡分离株是强毒株。该分析是在平均死亡时间和脑内致病性指数测试结果不一致之后进行的。通过建立核苷酸序列树状图，我们发现有争议的Ber-Tuvia分离株与已知的强毒株Herev-Laet明显属于同一组。Ber-Tuvia 92与Herev-Laet强毒株之间的差异为6%，而与中发型和缓发型分离株的差异大于16%。在融合蛋白裂解氨基酸序列中，Ber-Tuvia分离株在第112、113位以及115、116位分别含有Arg/Arg和Lys/Arg氨基酸，这是强毒NDV分离株的典型特征。
