Kumanan K, Mathivanan B, Vijayarani K, Gandhi A Arul, Ramadass P, Nachimuthu K
Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India.
Acta Virol. 2005;49(2):105-9.
Five Newcastle disease virus (NDV) isolates from pigeons were characterized by biological and molecular methods. Four of the five isolates were found to be velogenic with high intracerebral pathogenicity indices (ICPI). The fusion protein cleavage site (FPCS) sequences of these isolates had multiple basic amino acids RRQKRF at positions 112-116 and a phenyl alanine at position 117 characteristic of velogenic isolates. Three of these velogenic isolates were phylogenetically related to mesogenic vaccine virus strain and the fourth one to a few exotic velogenic isolates. The lentogenic isolate obtained in this study was identical with the LaSota strain.
采用生物学和分子方法对5株来自鸽子的新城疫病毒（NDV）分离株进行了鉴定。5株分离株中有4株为速发型，具有高脑内致病性指数（ICPI）。这些分离株的融合蛋白裂解位点（FPCS）序列在112-116位有多个碱性氨基酸RRQKRF，在117位有苯丙氨酸，这是速发型分离株的特征。其中3株速发型分离株在系统发育上与中等毒力疫苗病毒株相关，第4株与一些外来速发型分离株相关。本研究获得的缓发型分离株与LaSota株相同。
