Tissi L, von Hunolstein C, Bistoni F, Marangi M, Parisi L, Orefici G
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
J Med Microbiol. 1998 Aug;47(8):717-23. doi: 10.1099/00222615-47-8-717.
The ability of different serotypes of group B streptococci (GBS) to induce septic arthritis in mice was compared. Types II, III, IV, V, VI and VII GBS were investigated. A highly capsulate strain of type III GBS, COH1, and its mutants, COH1-11 (lacking capsular sialic acid) and COH1-13 (non-capsulate), obtained by transposon insertional mutagenesis, were used to assess the role of type-specific polysaccharide on the induction of arthritis. At an intravenous dose of 10(7) cfu/mouse, reference strains of types II, III, IV, VI and VII and type III strain COH1 induced arthritis with an incidence ranging from 70 to 90%. For type V and strain COH1-11, 10(8) cfu/mouse was required to obtain a 50% incidence of arthritis; lesions were not evident with strain COH1-13. The presence of the capsule played a major role in the induction of GBS septic arthritis. The presence and amount of sialic acid in capsular polysaccharide influenced the incidence of articular lesions. The bacterial dose affected the manifestations of arthritis; the less virulent strains of GBS also induced articular lesions when an adequate number of micro-organisms reached the joints.
比较了B族链球菌（GBS）不同血清型诱导小鼠感染性关节炎的能力。研究了II、III、IV、V、VI和VII型GBS。通过转座子插入诱变获得的III型GBS高荚膜菌株COH1及其突变体COH1-11（缺乏荚膜唾液酸）和COH1-13（无荚膜），用于评估型特异性多糖在关节炎诱导中的作用。静脉注射剂量为10(7) cfu/小鼠时，II、III、IV、VI和VII型参考菌株以及III型菌株COH1诱导关节炎的发生率为70%至90%。对于V型和菌株COH1-11，需要10(8) cfu/小鼠才能获得50%的关节炎发生率；菌株COH1-13未出现明显病变。荚膜的存在在GBS感染性关节炎的诱导中起主要作用。荚膜多糖中唾液酸的存在和含量影响关节病变的发生率。细菌剂量影响关节炎的表现；当足够数量的微生物到达关节时，GBS毒力较低的菌株也会诱导关节病变。
