Orlowski S, An D, Belehradek J, Mir L M
Laboratory of Physicochemistry and Pharmacology of Biological Macromolecules, UMR 1772 CNRS-Institut Gustave-Roussy, Villejuif, France.
Anticancer Drugs. 1998 Jul;9(6):551-6. doi: 10.1097/00001813-199807000-00006.
Murine Lewis lung (3LL) tumors are characterized by the appearance of lung metastases after a regular period following their s.c. transplantation. We tested the respective efficiencies of various antitumor treatments: (i) electrochemotherapy (ECT), i.e. the systemic injection of bleomycin associated with electric pulses, locally delivered, that permeabilizes the tumor cells; (ii) intratumoral injection of histoincompatible cells that have been engineered in vitro to secrete high amounts of interleukin-2; and (iii) the combination of these two treatments. The growth of the s.c. transplanted tumors was followed up and the number of lung metastases was counted 14 days after the treatment. ECT alone resulted in the reduction of both the size of the tumor and the number of lung metastases. This latter effect can be partially explained by the effects of ECT on the s.c. tumor mass from which 3LL cells escape to colonize the lungs. The injection of IL-2-secreting cells alone had no effect on the s.c. mass and only a limited effect on the number of lung metastases. However, the combined treatment ECT plus IL-2-secreting cells resulted in antimetastatic effects potentiation that could result from stimulation of a non-specific immune response through an increase of NK activity.
小鼠Lewis肺癌（3LL）肿瘤的特点是在皮下移植后的一段固定时间后会出现肺转移。我们测试了各种抗肿瘤治疗方法的各自效率：（i）电化学疗法（ECT），即全身注射博来霉素并结合局部施加的电脉冲，使肿瘤细胞通透性增加；（ii）瘤内注射在体外经过工程改造以分泌大量白细胞介素-2的组织不相容细胞；以及（iii）这两种治疗方法的联合。对皮下移植肿瘤的生长进行随访，并在治疗后14天计数肺转移灶的数量。单独使用ECT可导致肿瘤大小和肺转移灶数量均减少。后一种效应可以部分由ECT对皮下肿瘤块的作用来解释，3LL细胞从该肿瘤块中逸出并定植于肺部。单独注射分泌IL-2的细胞对皮下肿块没有影响，对肺转移灶数量只有有限的影响。然而，ECT加分泌IL-2细胞的联合治疗导致抗转移作用增强，这可能是通过增加NK活性刺激非特异性免疫反应所致。
