Kavanagh D A, Svehla G
Department of Dental Public Health, United Medical Dental Schools, London, UK.
Arch Oral Biol. 1998 Dec;43(12):1023-7. doi: 10.1016/s0003-9969(98)00085-5.
Calcium, phosphate and buffering capacity were measured in children's (n=43) unstimulated saliva between September 1990 and June 1991 inclusive. Saliva samples were collected in the afternoon, once a month, on 10 consecutive occasions. Buffering capacity was assessed immediately after sample collection using the Dentobuff strip method. The remainder of the saliva sample was then stored at -18 degrees C and, after thawing, total calcium and inorganic phosphate were measured. The intra-individual variation of buffering capacity over time was statistically significant (Friedman two-way ANOVA, p < 0.001). The intra-individual variability of salivary calcium was also statistically significant (repeated-measures ANOVA, p < 0.001). However, no evidence of a statistically significant variation in phosphate was observed. It is concluded that a combination of flow-rate fluctuation over time and the methods used to assess salivary constituents might have contributed to this variability.
在1990年9月至1991年6月（含）期间，对43名儿童的非刺激性唾液进行了钙、磷和缓冲能力的测量。唾液样本于下午采集，每月一次，连续采集10次。采集样本后立即使用Dentobuff试纸法评估缓冲能力。然后将其余的唾液样本储存在-18℃，解冻后测量总钙和无机磷。缓冲能力随时间的个体内变化具有统计学意义（Friedman双向方差分析，p<0.001）。唾液钙的个体内变异性也具有统计学意义（重复测量方差分析，p<0.001）。然而，未观察到磷有统计学显著变化的证据。得出的结论是，随时间的流速波动和用于评估唾液成分的方法可能导致了这种变异性。
