Blum B, Seebeck T, Braun R, Ferris P, Vogt V
Nucleic Acids Res. 1983 Dec 10;11(23):8519-33. doi: 10.1093/nar/11.23.8519.
We have used S1 nuclease to map the initiation site of ribosomal RNA transcription in the acellular slime mold Physarum polycephalum, and we have determined the sequence of 1011 nucleotides surrounding the start site. Consistent with others' observations, there is little homology with the comparable region of other species. As predicted by previous restriction mapping, direct repeats roughly 30 base pairs in length are present upstream of the initiation site and a 148 base pair duplication occurs in the external transcribed spacer. The results also suggest the presence of a processing site within the external transcribed spacer of the ribosomal transcription unit.
我们利用S1核酸酶来定位无细胞黏菌多头绒泡菌核糖体RNA转录的起始位点，并确定了起始位点周围1011个核苷酸的序列。与其他人的观察结果一致，该序列与其他物种的可比区域几乎没有同源性。正如先前限制酶切图谱所预测的那样，起始位点上游存在长度约为30个碱基对的直接重复序列，并且在外部转录间隔区发生了148个碱基对的重复。结果还表明，核糖体转录单位的外部转录间隔区内存在一个加工位点。
