Department of Periodontology, UCL Eastman Dental Institute for Oral Health Care Sciences University of London, 256 Gray's Inn Road, London, WC1X 8LD, UK.
Clin Oral Investig. 2012 Feb;16(1):239-49. doi: 10.1007/s00784-010-0478-7. Epub 2010 Oct 22.
Degree III furcation involvements were surgically created at four first molars in each of three monkeys. Following 6 weeks of healing, full-thickness flaps were elevated. Following 24% EDTA gel conditioning, the defects were treated with one of the following: (1) enamel matrix proteins (EMD), (2) guided tissue regeneration (GTR) or (3) a combination EMD and GTR. The control defects did not receive any treatment. After 5 months of healing, the animals were sacrificed. Three 8 μm thick histological central sections, 100 μm apart, were used for histomorphometric analysis in six zones of each tooth either within the furcation area or on the pristine external surface of the root. In all specimens, new cementum with inserting collagen fibres was formed. Following GTR or GTR + EMD, cementum was formed up to and including the furcation fornix indicating complete regeneration on the defect periphery. Periodontal ligament fibres were less in all four modalities compared to pristine tissues. In the teeth treated with GTR and GTR + EMD a higher volume of bone and periodontal ligament tissues was observed compared to EMD. After 5 months of healing, regenerated tissues presented quantitative differences from the pristine tissues. In the two modalities where GTR alone or combined with EMD was used, the regenerated tissues differed in quantity from the EMD-treated sites.
在三只猴子的每只四颗第一磨牙上造成 III 度分叉受累。经过 6 周的愈合，全厚瓣被掀起。经过 24% EDTA 凝胶处理后，用以下方法之一治疗这些缺损：（1）釉基质蛋白（EMD），（2）引导组织再生（GTR）或（3）EMD 和 GTR 的组合。对照缺损未接受任何治疗。愈合 5 个月后，处死动物。每只牙的分叉区或根的原始外表面的 6 个区域内，使用 3 个 8μm 厚的组织学中央切片，间隔 100μm，进行组织形态计量分析。在所有标本中，形成了具有插入胶原纤维的新牙骨质。在 GTR 或 GTR+EMD 之后，牙骨质形成直至分叉穹窿，表明缺损边缘完全再生。与原始组织相比，所有四种方式的牙周韧带纤维都较少。在用 GTR 和 GTR+EMD 治疗的牙齿中，观察到骨和牙周韧带组织的体积高于 EMD。愈合 5 个月后，再生组织与原始组织在数量上存在差异。在单独使用 GTR 或与 EMD 联合使用的两种方式中，再生组织在数量上与 EMD 治疗部位不同。
