Al-Saif N M, O'Neill G L, Magee J T, Brazier J S, Duerden B I
Department of Medical Microbiology and Public Health Laboratory, University of Wales College of Medicine, University Hospital of Wales, Cardiff.
J Med Microbiol. 1998 Feb;47(2):117-21. doi: 10.1099/00222615-47-2-117.
The relationships between environmental isolates of Clostridium difficile were examined by two typing methods, PCR ribotyping and pyrolysis mass spectrometry (PyMS). The 184 isolates were divided into 23 different PCR ribotypes, 13 of which were producers of toxins A and B; the remaining 10 types did not produce either toxin A or B. PyMS analysis resolved 31 groups with 60 (32.5%) isolates in one group (group 9). In both methods most of the isolates showed similar clustering. PCR ribotypes of the environmental isolates were compared with those of clinical isolates that had been typed previously. Seventeen PCR types (13 toxigenic PCR types and four non-toxigenic types) were found in both sets of isolates.
采用两种分型方法，即聚合酶链反应核糖体分型（PCR ribotyping）和热解质谱法（PyMS），对艰难梭菌的环境分离株之间的关系进行了研究。184株分离株被分为23种不同的PCR核糖体分型，其中13种是毒素A和毒素B的产生菌；其余10种不产生毒素A或毒素B。热解质谱分析分出31个组，其中一组（第9组）有60株（32.5%）分离株。在这两种方法中，大多数分离株显示出相似的聚类情况。将环境分离株的PCR核糖体分型与先前已分型的临床分离株的分型进行了比较。在两组分离株中均发现了17种PCR类型（13种产毒PCR类型和4种非产毒类型）。
