Koopmann E, Logemann E, Hahlbrock K
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Carl-von-Linné-Weg 10, D-50829 Köln, Germany.
Plant Physiol. 1999 Jan;119(1):49-56. doi: 10.1104/pp.119.1.49.
A previously isolated parsley (Petroselinum crispum) cDNA with high sequence similarity to cinnamate 4-hydroxylase (C4H) cDNAs from several plant sources was expressed in yeast (Saccharomyces cerevisiae) containing a plant NADPH:cytochrome P450 oxidoreductase and verified as encoding a functional C4H (CYP73A10). Low genomic complexity and the occurrence of a single type of cDNA suggest the existence of only one C4H gene in parsley. The encoded mRNA and protein, in contrast to those of a functionally related NADPH:cytochrome P450 oxidoreductase, were strictly coregulated with phenylalanine ammonia-lyase mRNA and protein, respectively, as demonstrated by coinduction under various conditions and colocalization in situ in cross-sections from several different parsley tissues. These results support the hypothesis that the genes encoding the core reactions of phenylpropanoid metabolism form a tight regulatory unit.
一个先前分离得到的、与来自多种植物的肉桂酸-4-羟化酶（C4H）cDNA具有高度序列相似性的欧芹（Petroselinum crispum）cDNA，在含有植物NADPH：细胞色素P450氧化还原酶的酵母（Saccharomyces cerevisiae）中表达，并被证实编码一种功能性C4H（CYP73A10）。低基因组复杂性和单一类型cDNA的出现表明欧芹中仅存在一个C4H基因。与功能相关的NADPH：细胞色素P450氧化还原酶的mRNA和蛋白质不同，所编码的mRNA和蛋白质分别与苯丙氨酸解氨酶的mRNA和蛋白质严格共调节，这在多种条件下的共诱导以及来自几种不同欧芹组织的横截面原位共定位中得到了证明。这些结果支持了这样的假设，即编码苯丙烷类代谢核心反应的基因形成一个紧密的调节单元。
