来自欧芹的肉桂酸4-羟化酶的调控与功能表达。
Regulation and functional expression of cinnamate 4-hydroxylase from parsley.
作者信息
Koopmann E, Logemann E, Hahlbrock K
机构信息
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Carl-von-Linné-Weg 10, D-50829 Köln, Germany.
出版信息
Plant Physiol. 1999 Jan;119(1):49-56. doi: 10.1104/pp.119.1.49.
Abstract
A previously isolated parsley (Petroselinum crispum) cDNA with high sequence similarity to cinnamate 4-hydroxylase (C4H) cDNAs from several plant sources was expressed in yeast (Saccharomyces cerevisiae) containing a plant NADPH:cytochrome P450 oxidoreductase and verified as encoding a functional C4H (CYP73A10). Low genomic complexity and the occurrence of a single type of cDNA suggest the existence of only one C4H gene in parsley. The encoded mRNA and protein, in contrast to those of a functionally related NADPH:cytochrome P450 oxidoreductase, were strictly coregulated with phenylalanine ammonia-lyase mRNA and protein, respectively, as demonstrated by coinduction under various conditions and colocalization in situ in cross-sections from several different parsley tissues. These results support the hypothesis that the genes encoding the core reactions of phenylpropanoid metabolism form a tight regulatory unit.
摘要
一个先前分离得到的、与来自多种植物的肉桂酸-4-羟化酶（C4H）cDNA具有高度序列相似性的欧芹（Petroselinum crispum）cDNA，在含有植物NADPH：细胞色素P450氧化还原酶的酵母（Saccharomyces cerevisiae）中表达，并被证实编码一种功能性C4H（CYP73A10）。低基因组复杂性和单一类型cDNA的出现表明欧芹中仅存在一个C4H基因。与功能相关的NADPH：细胞色素P450氧化还原酶的mRNA和蛋白质不同，所编码的mRNA和蛋白质分别与苯丙氨酸解氨酶的mRNA和蛋白质严格共调节，这在多种条件下的共诱导以及来自几种不同欧芹组织的横截面原位共定位中得到了证明。这些结果支持了这样的假设，即编码苯丙烷类代谢核心反应的基因形成一个紧密的调节单元。
相似文献
1
Regulation and functional expression of cinnamate 4-hydroxylase from parsley.来自欧芹的肉桂酸4-羟化酶的调控与功能表达。
Plant Physiol. 1999 Jan;119(1):49-56. doi: 10.1104/pp.119.1.49.
2
Differentially regulated NADPH:cytochrome P450 oxidoreductases in parsley.欧芹中差异调节的NADPH：细胞色素P450氧化还原酶
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14954-9. doi: 10.1073/pnas.94.26.14954.
3
Cinnamate-4-hydroxylase expression in Arabidopsis. Regulation in response to development and the environment.拟南芥中肉桂酸-4-羟化酶的表达。对发育和环境响应的调控。
Plant Physiol. 1997 Mar;113(3):729-38. doi: 10.1104/pp.113.3.729.
4
Isolation of a cDNA and a genomic clone encoding cinnamate 4-hydroxylase from Arabidopsis and its expression manner in planta.从拟南芥中分离出编码肉桂酸4-羟化酶的cDNA和基因组克隆及其在植物中的表达方式。
Plant Physiol. 1997 Mar;113(3):755-63. doi: 10.1104/pp.113.3.755.
5
Cinnamic acid 4-hydroxylase mechanism-based inactivation by psoralen derivatives: cloning and characterization of a C4H from a psoralen producing plant-Ruta graveolens-exhibiting low sensitivity to psoralen inactivation.补骨脂素衍生物对肉桂酸4-羟化酶的基于机制的失活作用：从对补骨脂素失活敏感性较低的产补骨脂素植物——芸香中克隆并鉴定一种肉桂酸4-羟化酶
Arch Biochem Biophys. 2004 Feb 1;422(1):71-80. doi: 10.1016/j.abb.2003.12.013.
6
Isolation and sequence of a cDNA encoding the Jerusalem artichoke cinnamate 4-hydroxylase, a major plant cytochrome P450 involved in the general phenylpropanoid pathway.编码菊芋肉桂酸4-羟化酶的cDNA的分离与测序，该酶是参与苯丙烷类通用途径的一种主要植物细胞色素P450。
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4102-6. doi: 10.1073/pnas.90.9.4102.
7
Colocalization of L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase for metabolic channeling in phenylpropanoid biosynthesis.苯丙氨酸解氨酶与肉桂酸4-羟化酶在苯丙烷类生物合成中的代谢通道化共定位
Plant Cell. 2004 Nov;16(11):3098-109. doi: 10.1105/tpc.104.024406. Epub 2004 Oct 7.
8
Functional characterization and subcellular localization of poplar (Populus trichocarpa x Populus deltoides) cinnamate 4-hydroxylase.毛果杨（Populus trichocarpa x Populus deltoides）肉桂酸4-羟化酶的功能表征及亚细胞定位
Plant Physiol. 2001 May;126(1):317-29. doi: 10.1104/pp.126.1.317.
9
Molecular cloning and sequencing of a cDNA encoding mung bean cytochrome P450 (P450C4H) possessing cinnamate 4-hydroxylase activity.编码具有肉桂酸4-羟化酶活性的绿豆细胞色素P450（P450C4H）的cDNA的分子克隆与测序
Biochem Biophys Res Commun. 1993 Feb 15;190(3):875-80. doi: 10.1006/bbrc.1993.1130.
10
Functional expression of cinnamate 4-hydroxylase from Ammi majus L.来自药用阿米芹的肉桂酸4-羟化酶的功能表达
Phytochemistry. 2003 Sep;64(2):445-52. doi: 10.1016/s0031-9422(03)00265-6.
引用本文的文献
1
Genome-wide characterization of PAL, C4H, and 4CL genes regulating the phenylpropanoid pathway in Vanilla planifolia.香草兰中调控苯丙烷类途径的苯丙氨酸解氨酶（PAL）、肉桂酸-4-羟化酶（C4H）和4-香豆酸:辅酶A连接酶（4CL）基因的全基因组特征分析
Sci Rep. 2025 Mar 28;15(1):10714. doi: 10.1038/s41598-024-81968-w.
2
Anabolic metabolism of autotoxic substance coumarins in plants.植物中自毒性物质香豆素的合成代谢。
PeerJ. 2023 Dec 6;11:e16508. doi: 10.7717/peerj.16508. eCollection 2023.
3
Targeted control of supporting pathways in paclitaxel biosynthesis with CRISPR-guided methylation.利用CRISPR引导的甲基化对紫杉醇生物合成中的支持途径进行靶向调控。
Front Bioeng Biotechnol. 2023 Oct 17;11:1272811. doi: 10.3389/fbioe.2023.1272811. eCollection 2023.
4
Functional characterization of Cinnamate 4-hydroxylase gene family in soybean (Glycine max).大豆（Glycine max）肉桂酸 4-羟化酶基因家族的功能特征。
PLoS One. 2023 May 15;18(5):e0285698. doi: 10.1371/journal.pone.0285698. eCollection 2023.
5
A Cinnamate 4-HYDROXYLASE1 from Safflower Promotes Flavonoids Accumulation and Stimulates Antioxidant Defense System in Arabidopsis.红花 4-香豆酸羟化酶 1 促进类黄酮积累并刺激拟南芥的抗氧化防御系统。
Int J Mol Sci. 2023 Mar 11;24(6):5393. doi: 10.3390/ijms24065393.
6
Comparative temporal metabolomics studies to investigate interspecies variation in three Ocimum species.比较时间代谢组学研究以调查三种罗勒属植物种间变异。
Sci Rep. 2020 Mar 23;10(1):5234. doi: 10.1038/s41598-020-61957-5.
7
Transcriptomic Analysis of Peels with a Focus on the Key Genes for Anthocyanin Biosynthesis.转录组分析果皮，重点关注花色苷生物合成的关键基因。
Int J Mol Sci. 2020 Feb 13;21(4):1245. doi: 10.3390/ijms21041245.
8
The Plant Growth-Promoting Fungus (PGPF) Alternaria sp. A13 Markedly Enhances Salvia miltiorrhiza Root Growth and Active Ingredient Accumulation under Greenhouse and Field Conditions.植物促生真菌（PGPF）链格孢菌 A13 在温室和田间条件下显著促进丹参根系生长和有效成分积累。
Int J Mol Sci. 2018 Jan 16;19(1):270. doi: 10.3390/ijms19010270.
9
Characterization and Expression Profiling of Camellia sinensis Cinnamate 4-hydroxylase Genes in Phenylpropanoid Pathways.茶树苯丙烷途径中肉桂酸4-羟化酶基因的表征与表达分析
Genes (Basel). 2017 Aug 1;8(8):193. doi: 10.3390/genes8080193.
10
RNAi down-regulation of cinnamate-4-hydroxylase increases artemisinin biosynthesis in Artemisia annua.RNA干扰下调肉桂酸-4-羟化酶可增加黄花蒿中青蒿素的生物合成。
Sci Rep. 2016 May 25;6:26458. doi: 10.1038/srep26458.
本文引用的文献
1
Phenylalanine ammonia lyase and cinnamic acid hydroxylases as assembled consecutive enzymes on microsomal membranes of cucumber cotyledons: Cooperation and subcellular distribution.苯丙氨酸解氨酶和肉桂酸羟化酶作为组装在黄瓜子叶微粒体膜上的连续酶：合作和亚细胞分布。
Planta. 1977 Jan;134(2):133-43. doi: 10.1007/BF00384962.
2
Responses of cultured parsley cells to elicitors from phytopathogenic fungi : timing and dose dependency of elicitor-induced reactions.培养的欧芹细胞对植物致病真菌激发子的反应：激发子诱导反应的时间和剂量依赖性
Plant Physiol. 1986 May;81(1):216-21. doi: 10.1104/pp.81.1.216.
3
Rapid Response of Suspension-cultured Parsley Cells to the Elicitor from Phytophthora megasperma var. sojae: INDUCTION OF THE ENZYMES OF GENERAL PHENYLPROPANOID METABOLISM.悬浮培养的欧芹细胞对来自大豆疫霉大豆变种激发子的快速反应：类苯丙烷代谢通用酶的诱导
Plant Physiol. 1981 Apr;67(4):768-73. doi: 10.1104/pp.67.4.768.
4
Host-Pathogen Interactions: X. Fractionation and Biological Activity of an Elicitor Isolated from the Mycelial Walls of Phytophthora megasperma var. sojae.植物病原菌互作：X. 从大豆疫霉菌菌核细胞壁中分离得到的激发子的分级和生物活性。
Plant Physiol. 1976 May;57(5):760-5. doi: 10.1104/pp.57.5.760.
5
Molecular Analysis and Heterologous Expression of an Inducible Cytochrome P-450 Protein from Periwinkle (Catharanthus roseus L.).长春花（Catharanthus roseus L.）中一种可诱导细胞色素P-450蛋白的分子分析与异源表达
Plant Physiol. 1992 Oct;100(2):998-1007. doi: 10.1104/pp.100.2.998.
6
Structure and elicitor or u.v.-light-stimulated expression of two 4-coumarate:CoA ligase genes in parsley.欧芹中两种 4-香豆酸：辅酶 A 连接酶基因的结构及激发子或紫外线刺激表达。
EMBO J. 1987 May;6(5):1189-95. doi: 10.1002/j.1460-2075.1987.tb02353.x.
7
Biphasic Temporal and Spatial Induction Patterns of Defense-Related mRNAs and Proteins in Fungus-Infected Parsley Leaves.真菌侵染的芹菜叶中防御相关mRNA和蛋白质的双相时空诱导模式
Plant Physiol. 1996 Sep;112(1):131-140. doi: 10.1104/pp.112.1.131.
8
Extensive reprogramming of primary and secondary metabolism by fungal elicitor or infection in parsley cells.真菌激发子或感染对芹菜细胞中初级和次级代谢的广泛重编程。
Biol Chem. 1998 Aug-Sep;379(8-9):1127-35. doi: 10.1515/bchm.1998.379.8-9.1127.
9
Two isoforms of NADPH:cytochrome P450 reductase in Arabidopsis thaliana. Gene structure, heterologous expression in insect cells, and differential regulation.拟南芥中NADPH：细胞色素P450还原酶的两种同工型。基因结构、在昆虫细胞中的异源表达及差异调控。
Plant Physiol. 1998 Jan;116(1):357-67. doi: 10.1104/pp.116.1.357.
10
Differentially regulated NADPH:cytochrome P450 oxidoreductases in parsley.欧芹中差异调节的NADPH：细胞色素P450氧化还原酶
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14954-9. doi: 10.1073/pnas.94.26.14954.
Zotero 插件