Abe H, Harada T, Kanehara M, Shimada T, Ohbayashi F, Oshiki T
Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Genes Genet Syst. 1998 Aug;73(4):237-42. doi: 10.1266/ggs.73.237.
In the silkworm, Bombyx mori, nonsusceptibility to B. mori densonucleosis virus type-1 (BmDNV-1) is controlled by a recessive gene, nsd-1 (nonsusceptibility to DNV-1), located on the twenty-first chromosome. We investigated genetic linkage between five random amplified polymorphic DNA (RAPD) markers and the +nsd-1 gene. Initially, we constructed the CSD-1 strain (nsd-1/+) which is congenic to strain C137 (nsd-1/nsd-1) for the twenty-first chromosome, starting with a female of C137 and a male of strain J137 (+nsd-1/+nsd-1). For the crossing over experiment, a female of C137 was crossed with a male (nsd-1/+) of CSD-1. Segregation analysis showed that the most closely linked RAPD marker mapped 3.0 cM distant from +nsd-1. A more specific marker for +nsd-1 was made by converting this RAPD band into a sequence characterized amplified region (SCAR) using a series of newly designed primer pairs based on its DNA sequence.
在家蚕（Bombyx mori）中，对家蚕1型浓核症病毒（BmDNV - 1）的不敏感性由位于第21号染色体上的隐性基因nsd - 1（对DNV - 1不敏感）控制。我们研究了五个随机扩增多态性DNA（RAPD）标记与+nsd - 1基因之间的遗传连锁关系。最初，我们构建了CSD - 1品系（nsd - 1/+），它在第21号染色体上与C137品系（nsd - 1/nsd - 1）同源，起始于C137品系的一只雌性蚕和J137品系（+nsd - 1/+nsd - 1）的一只雄性蚕。对于交叉实验，将C137品系的一只雌性蚕与CSD - 1品系的一只雄性蚕（nsd - 1/+）杂交。分离分析表明，与+nsd - 1连锁最紧密的RAPD标记位于距其3.0厘摩处。基于该RAPD条带的DNA序列，使用一系列新设计的引物对将其转化为序列特征性扩增区域（SCAR），从而获得了一个更特异的+nsd - 1标记。
