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本文引用的文献

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Organization of the Chorion Genes of BOMBYX MORI, a Multigene Family. II. Partial Localization of Three Gene Clusters.家蚕绒茧层多基因家族绒类基因的组织。Ⅱ.三个基因簇的部分定位。
Genetics. 1979 Aug;92(4):1173-85. doi: 10.1093/genetics/92.4.1173.
2
Linkage map for the Asian tiger mosquito [Aedes (Stegomyia) albopictus] based on SSCP analysis of RAPD markers.基于随机扩增多态性DNA标记的单链构象多态性分析构建的白纹伊蚊（亚洲虎蚊）连锁图谱。
J Hered. 1997 Nov-Dec;88(6):489-94. doi: 10.1093/oxfordjournals.jhered.a023142.
3
Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers.利用随机扩增多态性DNA标记的单链构象多态性分析对黄蜂（麦蛾茧蜂）和蚊子（埃及伊蚊）进行密集连锁图谱构建。
Genetics. 1996 Aug;143(4):1727-38. doi: 10.1093/genetics/143.4.1727.
4
Integrated genetic map of Anopheles gambiae: use of RAPD polymorphisms for genetic, cytogenetic and STS landmarks.冈比亚按蚊的综合遗传图谱：利用随机扩增多态性DNA（RAPD）多态性构建遗传、细胞遗传和序列标签位点（STS）标记
Genetics. 1996 Jun;143(2):953-60. doi: 10.1093/genetics/143.2.953.
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An integrated genetic map of the African human malaria vector mosquito, Anopheles gambiae.非洲人类疟疾媒介冈比亚按蚊的综合遗传图谱。
Genetics. 1996 Jun;143(2):941-52. doi: 10.1093/genetics/143.2.941.
6
Identification, mapping and linkage analysis of randomly amplified DNA polymorphisms in Tetrahymena thermophila.嗜热四膜虫随机扩增多态性DNA的鉴定、定位及连锁分析
Genetics. 1996 Jun;143(2):811-21. doi: 10.1093/genetics/143.2.811.
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Linkage map for Aedes aegypti using restriction fragment length polymorphisms.利用限制性片段长度多态性构建的埃及伊蚊连锁图谱。
J Hered. 1993 Jul-Aug;84(4):241-7. doi: 10.1093/oxfordjournals.jhered.a111333.
8
Polymorphism and linkage analysis of the prothoracicotropic hormone gene in the silkmoth, Bombyx mori.家蚕促前胸腺激素基因的多态性与连锁分析
Genet Res. 1994 Jun;63(3):189-95. doi: 10.1017/s0016672300032341.
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A 300 kilobase interval genetic map of rice including 883 expressed sequences.一张包含883个表达序列的300千碱基水稻区间遗传图谱。
Nat Genet. 1994 Dec;8(4):365-72. doi: 10.1038/ng1294-365.
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Linkage map of the honey bee, Apis mellifera, based on RAPD markers.基于随机扩增多态性DNA（RAPD）标记的蜜蜂（西方蜜蜂）连锁图谱。
Genetics. 1995 Mar;139(3):1371-82. doi: 10.1093/genetics/139.3.1371.

基于1018个分子标记构建的覆盖家蚕所有染色体的高密度遗传图谱。

A dense genetic map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers.

作者信息

Yasukochi Y

机构信息

National Institute of Sericultural and Entomological Science (NISES), Tsukuba, Ibaraki 305-8634, Japan.

出版信息

Genetics. 1998 Dec;150(4):1513-25. doi: 10.1093/genetics/150.4.1513.

DOI:10.1093/genetics/150.4.1513
PMID:9832528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1460425/
Abstract

A dense linkage map was constructed for the silkworm, Bombyx mori, containing 1018 genetic markers on all 27 autosomes and the Z chromosome. Most of the markers, covering approximately 2000 cM, were randomly amplified polymorphic DNAs amplified with primer-pairs in combinations of 140 commercially available decanucleotides. In addition, eight known genes and five visible mutations were mapped. Bombyx homologues of engrailed and invected genes were found to be closely linked, as in Drosophila melanogaster. The average interval between markers was approximately 2 cM, equal to approximately 500 kb. The correspondence of seven linkage groups to counterparts of the conventional linkage map was determined. This map is the first linkage map in insects having a large number of chromosomes (n = 28) that covers all chromosomes without any gaps.

摘要

构建了家蚕的高密度连锁图谱，该图谱包含27条常染色体和Z染色体上的1018个遗传标记。大多数标记覆盖约2000厘摩，是用140种市售十聚体组合的引物对随机扩增的多态性DNA。此外，还定位了8个已知基因和5个可见突变。发现家蚕中engrailed和invected基因的同源物紧密连锁，如同在黑腹果蝇中一样。标记之间的平均间隔约为2厘摩，相当于约500千碱基对。确定了7个连锁群与传统连锁图谱对应物的对应关系。该图谱是昆虫中第一张覆盖所有染色体且无任何间隙的、具有大量染色体（n = 28）的连锁图谱。