Yasukochi Y
National Institute of Sericultural and Entomological Science (NISES), Tsukuba, Ibaraki 305-8634, Japan.
Genetics. 1998 Dec;150(4):1513-25. doi: 10.1093/genetics/150.4.1513.
A dense linkage map was constructed for the silkworm, Bombyx mori, containing 1018 genetic markers on all 27 autosomes and the Z chromosome. Most of the markers, covering approximately 2000 cM, were randomly amplified polymorphic DNAs amplified with primer-pairs in combinations of 140 commercially available decanucleotides. In addition, eight known genes and five visible mutations were mapped. Bombyx homologues of engrailed and invected genes were found to be closely linked, as in Drosophila melanogaster. The average interval between markers was approximately 2 cM, equal to approximately 500 kb. The correspondence of seven linkage groups to counterparts of the conventional linkage map was determined. This map is the first linkage map in insects having a large number of chromosomes (n = 28) that covers all chromosomes without any gaps.
构建了家蚕的高密度连锁图谱，该图谱包含27条常染色体和Z染色体上的1018个遗传标记。大多数标记覆盖约2000厘摩，是用140种市售十聚体组合的引物对随机扩增的多态性DNA。此外，还定位了8个已知基因和5个可见突变。发现家蚕中engrailed和invected基因的同源物紧密连锁，如同在黑腹果蝇中一样。标记之间的平均间隔约为2厘摩，相当于约500千碱基对。确定了7个连锁群与传统连锁图谱对应物的对应关系。该图谱是昆虫中第一张覆盖所有染色体且无任何间隙的、具有大量染色体（n = 28）的连锁图谱。
