Beyersdorf-Radeck B, Riedel K, Karlson U, Bachmann T T, Schmid R D
GBF, Braunschweig, Germany.
Microbiol Res. 1998 Nov;153(3):239-45. doi: 10.1016/S0944-5013(98)80006-2.
A screening device based on microorganisms immobilised onto a Clark-type oxygen electrode was used to monitor the potential of these microorganisms for the degradation and detection of xenobiotic compounds especially their chlorinated derivatives. The sensitivity and specificity of various species of Pseudomonas, Sphinomonas, Ralstonia, Rhodococcus were characterised in relation to xenobiotic compounds by using biosensor techniques. The following groups of xenobiotics were subjects of investigation: chlorophenols, chlorobenzoates, 2,4-D, PCB, dibenzofurane and their putative intermediates. Using this simple setup it proved possible to screen microbial strains for their potential to catabolize aromatic and chloroaromatic compounds under oxygen consumption. In a kinetic regime, a reproducible signal was obtained within minutes. Based on these results the sensor technique was a suitable method for the rapid characterization of microorganisms and allowed to gather information about the substrate spectrum.
一种基于固定在克拉克型氧电极上的微生物的筛选装置，用于监测这些微生物降解和检测外源化合物尤其是其氯化衍生物的潜力。利用生物传感器技术，对各种假单胞菌属、鞘氨醇单胞菌属、罗尔斯通氏菌属、红球菌属微生物针对外源化合物的敏感性和特异性进行了表征。以下几类外源化合物是研究对象：氯酚、氯苯酸盐、2,4-滴、多氯联苯、二苯并呋喃及其假定中间体。使用这种简单的装置，证明可以筛选微生物菌株在耗氧情况下分解代谢芳香族和氯代芳香族化合物的潜力。在动力学条件下，几分钟内就能获得可重复的信号。基于这些结果，传感器技术是快速表征微生物的合适方法，并能够收集有关底物谱的信息。
