Shlebak A A, Roberts I A, Stevens T A, Syzdlo R M, Goldman J M, Gordon M Y
Department of Haematology, Imperial College School of Medicine, Hammersmith Hospital, London.
Br J Haematol. 1998 Dec;103(4):1167-71. doi: 10.1046/j.1365-2141.1998.01093.x.
We investigated the impact of maternal and fetal variables on cord blood (CB) haemopoietic stem/progenitor cell content. These included maternal age, ethnic origin, parity, ABO and Rhesus D blood group, antenatal haemoglobin, alcohol and cigarette consumption at time of registration, mode of delivery, duration of the first and second stages of labour, gestational age, birth weight, cord pH and cord erythrocyte mean cell volume (MCV). Cord volumes and total nucleated cellularities (TNC) were recorded, the colony assay for granulocyte-macrophage colony-forming-cells (CFU-GM) was used to quantify the progenitor cells and the potential of CFU-GM to produce secondary colonies on replating was used as a measure of progenitor cell quality. We found: (1) significantly greater (P=0.04) volumes were collected from babies who weighed > or = 2.5kg versus babies with a birth weight <2.5kg; (2) significantly greater numbers of mononuclear cells (MNC) from mothers who drank 0-3 units versus those who drank > or = 4 units of alcohol weekly (P=0.03), and in babies with a cord pH < or = 7.1 v > 7.1 (P=0.02); (3) Significantly greater numbers of cord CFU-GM in mothers who drank 0-3 v > or = 4 units weekly (P=0.004) and smokers of > or = 10 v 0-9 cigarettes daily (P=0.02) and in spontaneous vaginal deliveries than assisted vaginal and caesarean deliveries (P=0.04), and (4) the potential of CFU-GM to produce secondary colonies was significantly greater in CB derived from Caucasians than from non-Caucasians ( P=0.02); in assisted vaginal delivery v spontaneous vaginal (P=0.02) and in deliveries with prolonged first stage of labour v short first stage of labour (P=0.04). We conclude that antenatal and perinatal variables may influence the CB stem/progenitor cell yield available for transplantation.
我们研究了母体和胎儿变量对脐血造血干/祖细胞含量的影响。这些变量包括产妇年龄、种族、胎次、ABO和RhD血型、产前血红蛋白水平、登记时的酒精和香烟消费量、分娩方式、第一产程和第二产程的时长、孕周、出生体重、脐血pH值和脐血红细胞平均体积（MCV）。记录了脐血体积和总核细胞数（TNC），采用粒细胞-巨噬细胞集落形成细胞（CFU-GM）集落分析法对祖细胞进行定量，并将CFU-GM再次接种时产生次级集落的能力作为祖细胞质量的一项指标。我们发现：（1）出生体重≥2.5kg的婴儿所采集的脐血体积显著大于出生体重<2.5kg的婴儿（P = 0.04）；（2）每周饮酒0 - 3单位的母亲所产婴儿的单核细胞（MNC）数量显著多于每周饮酒≥4单位的母亲所产婴儿（P = 0.03），且脐血pH值≤7.1的婴儿的MNC数量显著多于脐血pH值>7.1的婴儿（P = 0.02）；（3）每周饮酒0 - 3单位的母亲所产婴儿的脐血CFU-GM数量显著多于每周饮酒≥4单位的母亲所产婴儿（P = 0.004），每日吸烟≥10支的母亲所产婴儿的脐血CFU-GM数量显著多于每日吸烟0 - 9支的母亲所产婴儿（P = 0.02），且自然阴道分娩的婴儿的脐血CFU-GM数量显著多于辅助阴道分娩和剖宫产的婴儿（P = 0.04）；（4）来自白种人的脐血中CFU-GM产生次级集落的能力显著大于来自非白种人的脐血（P = 0.02）；辅助阴道分娩的脐血中CFU-GM产生次级集落的能力显著小于自然阴道分娩的脐血（P = 0.02），第一产程延长的分娩的脐血中CFU-GM产生次级集落的能力显著小于第一产程短的分娩的脐血（P = 0.04）。我们得出结论，产前和围产期变量可能会影响可用于移植的脐血干/祖细胞产量。
