Saudi Stem Cell Donor Registry and Cord Blood Bank, King Abdullah International Medical Research Center, College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.
Cellular Therapy Services, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.
Cell Tissue Bank. 2024 Jun;25(2):541-547. doi: 10.1007/s10561-023-10111-2. Epub 2023 Oct 15.
Today cord blood (CB) is a valuable source of hematopoietic stem cells to treat many hematological disorders. One of the limitations of CB utilization is the reduced number of nucleated cells including stem cells. Therefore, CB banks around the world have developed strategies in an attempt to improve donor selection and the quality of the CB inventory. This study aimed to determine the impact of passive smoking and caffeine consumption on CB quality. CBs were obtained from mothers who gave birth at King Abdulaziz Medical City. All mothers gave their informed consent. Personal interviews about the mother's demographics, smoking status and exposure, and caffeine consumption executed, followed by a chart review to analyze maternal and neonatal factors. Laboratory testing was performed on all collected CB units. Using descriptive statistics, maternal and newborn factors were analyzed. T-test or Mann-Whitney U Test, as appropriate, for continuous variables analysis to study the effect of second hand smoking and coffee consumption for the primary outcome. Our study demonstrated a reduction in CB MNC, including lymphocytes, in caffeine consumers among pregnant donors, as well as a reduction in cell potency activities, including total CFU and BFU-E. The effect of passive cigarette smoking on the same cohort was insignificant. Outcome of this study will help in optimizing the quality and quantity of stem cell harvesting from CB to get the maximum benefit and such knowledge will raise the awareness among pregnant women.
目前，脐带血（CB）是治疗许多血液系统疾病的造血干细胞的宝贵来源。CB 利用的一个限制因素是包括干细胞在内的有核细胞数量减少。因此，世界各地的 CB 库都制定了策略，试图改善供体选择和 CB 库存的质量。本研究旨在确定被动吸烟和咖啡因摄入对 CB 质量的影响。CB 从在阿卜杜勒阿齐兹国王医疗城分娩的母亲那里获得。所有母亲都表示知情同意。对母亲的人口统计学、吸烟状况和暴露情况以及咖啡因摄入情况进行了个人访谈，然后进行图表审查以分析母婴因素。对所有收集的 CB 单位进行实验室检测。使用描述性统计，分析了母婴和新生儿因素。使用 t 检验或曼-惠特尼 U 检验（视情况而定）分析连续变量，以研究二手烟和咖啡消费对主要结果的影响。我们的研究表明，在咖啡因消费者中，孕妇 CB 中的 MNC 减少，包括淋巴细胞，以及细胞活力活动减少，包括总 CFU 和 BFU-E。同一队列中被动吸烟的影响并不显著。本研究的结果将有助于优化从 CB 中采集干细胞的质量和数量，以获得最大的益处，这种知识将提高孕妇的意识。
