Hinson J P, Harwood S, Dawnay A B
Department of Biochemistry, St Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College.
Endocr Res. 1998 Aug-Nov;24(3-4):721-4. doi: 10.3109/07435809809032675.
The present study was designed to investigate the effect of ACTH on release of OLC from the intact isolated rat adrenal, perfused in situ. OLC was measured by EIA. The limit of detection of the assay was 23 pmol/10 minutes. Basal levels of OLC varied from 240 to <23 pmol/10 min. Basal corticosterone levels were generally higher than OLC while aldosterone were generally lower. In 3 of the 5 perfusions the addition of ACTH was followed by rapid increases in both OLC and corticosterone secretion rates within 10 minutes of stimulation. Stimulated levels of OLC were 2 to 4-fold and of corticosterone were 3 to 7-fold those found in basal samples. OLC was found to co-elute with authentic ouabain under isocratic HPLC analysis whilst perfusion medium itself contained no detectable OLC immunoreactivity. These preliminary data suggest that the intact perfused rat adrenal preparation is a useful model for investigating the acute regulation of OLC secretion.
本研究旨在探讨促肾上腺皮质激素（ACTH）对原位灌注的完整分离大鼠肾上腺释放哇巴因样化合物（OLC）的影响。通过酶免疫分析（EIA）测定OLC。该检测方法的检测限为23 pmol/10分钟。OLC的基础水平在240至<23 pmol/10分钟之间变化。基础皮质酮水平通常高于OLC，而醛固酮水平通常低于OLC。在5次灌注中的3次中，添加ACTH后，在刺激后10分钟内OLC和皮质酮分泌率迅速增加。刺激后的OLC水平是基础样本中水平的2至4倍，皮质酮水平是基础样本中水平的3至7倍。在等度高效液相色谱分析中，发现OLC与纯哇巴因共洗脱，而灌注介质本身未检测到OLC免疫反应性。这些初步数据表明，完整灌注的大鼠肾上腺制备物是研究OLC分泌急性调节的有用模型。
