Schaumann R, Krosing J, Shah P M
Zentrum der Inneren Medizin, Medizinische Klinik III / Infektiologie, Klinikum der Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, D-60590 Frankfurt/Main, Germany.
Eur J Med Res. 1998 Dec 16;3(12):546-8.
To study the influence of human immunodeficiency virus (HIV) infection on phagocytosis of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by polymorphonuclear neutrophils (PMN) from HIV-infected patients in vitro. PMN were isolated from the blood of 25 HIV-infected patients (group 1: CD subset4 </= 250/microL: 14 patients, group 2: CD subset4 >250/microL: 11 patients ) by Percoll density gradient and incubated with E. coli and S. aureus. Subculture technique was used to determine PMN function at 0, 0.5, 1, 2, 4, 6, and 24 hours. Controls were run with PMN from healthy volunteers. Phagocytosis of E.coli and S.aureus by PMN of HIV-infected patients was significantly lower in group 1 (p <0.05). Reduced phagocytosis of E. coli and S. aureus was found in HIV-infected patients with low CD subset4-cell counts. Our findings may contribute to increased susceptibility to bacterial infection in HIV-infected patients with low CD subset4-cell counts.
为了在体外研究人类免疫缺陷病毒（HIV）感染对HIV感染患者多形核中性粒细胞（PMN）吞噬大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的影响。通过Percoll密度梯度从25例HIV感染患者（第1组：CD4亚群≤250/μL：14例患者，第2组：CD4亚群>250/μL：11例患者）的血液中分离出PMN，并与大肠杆菌和金黄色葡萄球菌一起孵育。采用传代培养技术在0、0.5小时、1、2、4、6和24小时测定PMN功能。用健康志愿者的PMN作为对照。在第1组中，HIV感染患者的PMN对大肠杆菌和金黄色葡萄球菌的吞噬作用显著降低（p<0.05）。在CD4亚群细胞计数低的HIV感染患者中发现对大肠杆菌和金黄色葡萄球菌的吞噬作用降低。我们的研究结果可能有助于解释CD4亚群细胞计数低的HIV感染患者对细菌感染易感性增加的原因。
