Réglier-Poupet H, Hakim J, Gougerot-Pocidalo M A, Elbim C
Laboratoire d'Immunologie et d'Hématologie, CHU Xavier-Bichat, 46, rue Henri-Huchard, 75877 Paris Cedex 18, France. Tel: (+33) 1 40 25 85 21, Fax: (+33) 1 40 25 88 53.
Eur Cytokine Netw. 1998 Dec;9(4):633-8.
Cytokines such as IL-10, IL-4, IL-13 and TGF-beta play a major role in the regulation of immune responses and are considered as anti-inflammatory agents mainly due to their actions on monocytes. These cytokines are also known to participate in the regulation of PMN activities. However, few and contradictory results have been reported on their direct and priming effects on the PMN oxidative burst, which is essential for killing bacteria. We used a flow cytometry method to study the effects of these cytokines on the PMN oxidative burst; we also used whole blood to avoid PMN activation related to isolation procedures and in order to simulate the in vivo situation more closely. None of the cytokines tested had direct or priming effects on PMN H2O2 production. We also show for the first time that these cytokines do not modulate TNF priming of the PMN oxidative burst in response to N-formyl peptides (fMLP). These results show that the anti-bacterial activity of PMN, in terms of the PMN respiratory burst, is not down regulated by these anti-inflammatory cytokines in whole blood.
白细胞介素-10、白细胞介素-4、白细胞介素-13和转化生长因子-β等细胞因子在免疫反应调节中起主要作用，主要因其对单核细胞的作用而被视为抗炎因子。这些细胞因子也参与中性粒细胞活性的调节。然而，关于它们对中性粒细胞氧化爆发（这对杀灭细菌至关重要）的直接和启动作用，报道甚少且相互矛盾。我们采用流式细胞术方法研究这些细胞因子对中性粒细胞氧化爆发的影响；我们还使用全血以避免与分离程序相关的中性粒细胞激活，并更紧密地模拟体内情况。所测试的细胞因子均对中性粒细胞过氧化氢生成无直接或启动作用。我们还首次表明，这些细胞因子不会调节中性粒细胞对N-甲酰肽（fMLP）的氧化爆发的肿瘤坏死因子启动作用。这些结果表明，就中性粒细胞呼吸爆发而言，全血中的这些抗炎细胞因子不会下调中性粒细胞的抗菌活性。
