Luty A J, Lell B, Schmidt-Ott R, Lehman L G, Luckner D, Greve B, Matousek P, Herbich K, Schmid D, Ulbert S, Migot-Nabias F, Dubois B, Deloron P, Kremsner P G
University of Tübingen, Institute for Tropical Medicine, Department of Parasitology, Wilhelmstrasse 27, 72074 Tübingen, Germany. Tel: (+49) 7071 2980228, Fax: (+49) 7071 295189, e-mail: adrian.
Eur Cytokine Netw. 1998 Dec;9(4):639-46.
Using strict inclusion criteria, we conducted a hospital-based, case-control study in which 100 Gabonese children with severe Plasmodium falciparum malaria were matched for age, gender and provenance with 100 children presenting with mild malaria. Parasite antigen-specific cellular and humoral immunological responses were measured and compared with post-treatment parasite clearance times in each group. Significantly faster parasite clearance times were associated with in vitro production of IL-10 by acute-phase peripheral blood mononuclear cells (PBMC) in response to both liver and asexual stage parasite antigens, but not with proliferative, IFN-gamma, or TNF responses to the same antigens. In addition, in those children with mild malaria, higher levels of acute-phase antibody responses to liver stage antigen-1 (LSA-1) were associated with faster parasite clearance times, and were correlated with the presence of IL-10 responses to the same antigen. No such associations were found for IL-10 or antibody responses to a range of asexual blood stage antigens. Those with severe malaria had significantly lower levels of anti-LSA-1 antibodies compared to their counterparts with mild malaria. In conclusion, the results of this study suggest that parasite antigen-specific IL-10-mediated antibody responses may play a role in the control of asexual stage parasite multiplication in P. falciparum malaria.
我们采用严格的纳入标准，开展了一项基于医院的病例对照研究，将100名患有严重恶性疟原虫疟疾的加蓬儿童，按照年龄、性别和来源与100名患有轻度疟疾的儿童进行匹配。检测了寄生虫抗原特异性细胞免疫和体液免疫反应，并与每组治疗后的寄生虫清除时间进行比较。寄生虫清除时间显著加快与急性期外周血单核细胞（PBMC）在体外对肝脏期和无性期寄生虫抗原产生白细胞介素-10（IL-10）有关，但与对相同抗原的增殖反应、γ干扰素（IFN-γ）或肿瘤坏死因子（TNF）反应无关。此外，在那些患有轻度疟疾的儿童中，对肝脏期抗原-1（LSA-1）的急性期抗体反应水平较高与寄生虫清除时间较快有关，并且与对相同抗原的IL-10反应的存在相关。对于一系列无性血液期抗原的IL-10或抗体反应未发现此类关联。与患有轻度疟疾的儿童相比，患有严重疟疾的儿童抗LSA-1抗体水平显著较低。总之，本研究结果表明，寄生虫抗原特异性IL-10介导的抗体反应可能在控制恶性疟原虫疟疾无性期寄生虫增殖中发挥作用。
