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[荷瘤大鼠肝脏过氧化氢酶合成速率的转录后调控因素]

[Factors of post-transcription regulation of the rate of liver catalase synthesis in tumor-bearing rats].

作者信息

Komov V P, Rakhmanina T F, Bulat A D

出版信息

Biull Eksp Biol Med. 1976 Mar;81(3):303-5.

PMID:989027
Abstract

The factors-regulators of post-transcriptional regulation of the rate of catalase synthesis in the rat liver were studied under normal conditions and in carcinogenesis (the 2nd, 7th and the 14th days after the transplantation of Pliss lymphosarcoma). Factors Fact and Fing were purified from the supernatant and PH5-supernatant fractions of the rat liver by means of chromatography and rechromatography on DEAE-cellulose. A simple method of determination of the biological activity of these factors in vivo was proposed. Synthesis of catalase took place when (see article) (the 2nd and 14th days after the inosulation). The enzyme synthesis was absent when (see article) (7th day after the inoculation).

摘要

在正常条件下以及致癌过程中(移植普利斯氏淋巴肉瘤后的第2天、第7天和第14天),对大鼠肝脏中过氧化氢酶合成速率的转录后调控因子进行了研究。通过在DEAE - 纤维素上进行色谱法和再色谱法,从大鼠肝脏的上清液和PH5 - 上清液组分中纯化出因子Fact和Fing。提出了一种在体内测定这些因子生物活性的简单方法。当(见文章)(接种后的第2天和第14天)时发生过氧化氢酶的合成。当(见文章)(接种后的第7天)时酶合成不存在。

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