Guerrieri F, Vendemiale G, Grattagliano I, Cocco T, Pellecchia G, Altomare E
Institute of Medical Biochemistry and Chemistry and Centre for the Study of Mitochondria and Energy Metabolism (C.N.R.), University of Bari, Italy.
Free Radic Biol Med. 1999 Jan;26(1-2):34-41. doi: 10.1016/s0891-5849(98)00145-2.
Mitochondria, isolated from rat livers during the early phase of liver regeneration (7-24 h after partial hepatectomy), show: (i) decrease in the rate of ATP synthesis; (ii) increase of malondialdehyde and of oxidized protein production; (iii) decrease of the content of intramitochondrial glutathione and of protein thiols on mitochondrial proteins; (iv) increase of the glutathione bound to mitochondrial proteins by disulfide bonds. These observations suggest an increase of production of oxygen radicals in liver mitochondria, following partial hepatectomy, which can alter the function of the enzymes involved in the oxidative phosphorylation. Blue-native gel electrophoresis of rat liver mitochondria, isolated after partial hepatectomy, shows, during the early phase of liver regeneration (0-24 h after partial hepatectomy), a progressive decrease of the content of F0F1-ATP synthase complex. The amount of glutathione bound to the F0F1-ATP synthase, electroeluted from the blue-native gels, progressively increased during the early phase of liver regeneration. It is concluded that partial hepatectomy causes mitochondrial oxidative stress that, in turn, modifies proteins (such as F0F1-ATP synthase) involved in the mitochondrial oxidative phosphorylation.
从肝再生早期（部分肝切除术后7 - 24小时）的大鼠肝脏中分离出的线粒体表现出：（i）ATP合成速率降低；（ii）丙二醛及氧化蛋白生成增加；（iii）线粒体内谷胱甘肽含量及线粒体蛋白上蛋白质巯基减少；（iv）通过二硫键与线粒体蛋白结合的谷胱甘肽增加。这些观察结果表明，部分肝切除术后肝脏线粒体中氧自由基生成增加，这可能会改变参与氧化磷酸化的酶的功能。对部分肝切除后分离出的大鼠肝脏线粒体进行蓝色非变性凝胶电泳分析显示，在肝再生早期（部分肝切除术后0 - 24小时），F0F1 - ATP合酶复合体的含量逐渐降低。从蓝色非变性凝胶上电洗脱得到的与F0F1 - ATP合酶结合的谷胱甘肽量在肝再生早期逐渐增加。得出的结论是，部分肝切除术会导致线粒体氧化应激，进而改变参与线粒体氧化磷酸化的蛋白质（如F0F1 - ATP合酶）。
