Niewiadomska H, Mirowski M, Stempien M, Olborski B, Blonski J Z, Hanausek M, Wierzbicki R
Department of Oncology, Medical University, Lodz, Poland.
Neoplasma. 1998;45(4):216-22.
Paraffin-embedded tissue slides from 89 infiltrating ductal breast carcinoma, 10 fibrocystic disease and 10 fibroadenoma were assessed immunohistochemically using monoclonal antibodies against human p65 antigen and polyclonal antibodies against p65-like protein present in fetal bovine serum. We did not find any evident differences in p65 detection by polyclonal and monoclonal antibodies, however, monoclonal antibody seems to be more specific. This factor is not induced by cellular proliferation associated with nonneoplastic diseases what was confirmed by immunohistochemical analysis of expression of p65 protein and well know markers of proliferation (proliferating cell nuclear antigen--PCNA and Ki67). It was established that there is no correlation between p65 and PCNA or Ki67 expression. High proliferating indexes (PI) for PCNA (PI-PCNA) or Ki67 (PI-Ki67) may help in selection of tumors with high proliferating activity independently from histological grade of malignancy established by routine methods. The estimation of p65 protein may be useful in the selection of precancerous changes and more differentiated ductal cancer of the breast what raises the possibility that p65 antigen may be helpful in the screening examination of women with high risk for cancer development.
使用抗人p65抗原的单克隆抗体和抗胎牛血清中p65样蛋白的多克隆抗体，对89例浸润性导管乳腺癌、10例纤维囊性疾病和10例纤维腺瘤的石蜡包埋组织切片进行免疫组织化学评估。我们未发现多克隆抗体和单克隆抗体在p65检测上有任何明显差异，然而，单克隆抗体似乎更具特异性。通过对p65蛋白表达以及众所周知的增殖标志物（增殖细胞核抗原——PCNA和Ki67）的免疫组织化学分析证实，该因子并非由与非肿瘤性疾病相关的细胞增殖所诱导。已确定p65与PCNA或Ki67表达之间无相关性。PCNA（PI-PCNA）或Ki67（PI-Ki67）的高增殖指数（PI）可能有助于独立于常规方法确定的恶性组织学分级来选择具有高增殖活性的肿瘤。p65蛋白的评估可能有助于选择癌前病变和分化程度更高的乳腺导管癌，这增加了p65抗原可能有助于对癌症发生高危女性进行筛查检查的可能性。
