Moore S, Friedman R J, Singal D P, Gauldie J, Blajchman M A, Roberts R S
Thromb Haemost. 1976 Feb 29;35(1):70-81.
We have previously shown that repeated or continuous intimal injury caused by an indwelling aortic catheter causes a variety of lesions in rabbits maintained on a diet unsupplemented by lipid. These include fatty streaks, lipid-free fibrous plaques and lipid-rich raised thromboatherosclerotic plaques. Whether lipid-rich raised lesions are a result of injury or co-existing thrombosis or both is not clear. The present experiment was designed to answer this question. Anti-platelet serum (APS) to washed sonicated rabbit platelets was raised in sheep. PE 60 polyethylene catheters were placed in the aortas of 35 rabbits by way of a femoral artery. The animals were randomly divided into 2 groups. The experimental group (17 rabbits) received an intravascular injection of 1.0 ml of APS followed 8 hours later by a subcutaneous injection of 0.5 ml. Thereafter, 0.5 ml APS was given subcutaneously each day for 13 additional days. The control group (18 rabbits) received no APS. Platelet counts were done prior to surgery, at 5 minutes following surgery, at 4 days, 8 days and just prior to killing. Extent of lesions was estimated by photographing the opened aortas, projecting the photographs on cardboard, cutting out the areas occupied by the different lesions and weighing the cardboard. The mean weight of raised lesions in the control group was 6 to 7 times greater than in the experimental groups. Statistical analysis of this difference based on Welsh's "t" test for unequal variances was highly significant (P less than 0.001). Platelet counts in the experimental groups varied from 0 to 20,000 at 14 days. In animals with platelet counts less than or equal to 1,000 mm3 raised lesions were completely prevented. In a second experiment the effect of APS was compared with normal sheep serum (NSS). A similarly significant inhibition of raised lesions occurred in the APS group. The extent of lesions in the NSS control was similar to that in the No-APS group of the first experiment. These findings indicate that thrombosis is more important than injury in the development of lipid-rich raised lesions.
我们之前已经表明，留置主动脉导管引起的反复或持续性内膜损伤会在未补充脂质饮食的兔子身上引发多种病变。这些病变包括脂肪条纹、无脂质纤维斑块和富含脂质的隆起血栓性动脉粥样硬化斑块。富含脂质的隆起病变是损伤的结果还是同时存在的血栓形成的结果，或者两者皆是，尚不清楚。本实验旨在回答这个问题。用绵羊制备了针对洗涤并超声处理过的兔血小板的抗血小板血清（APS）。通过股动脉将PE 60聚乙烯导管置入35只兔子的主动脉。这些动物被随机分为两组。实验组（17只兔子）血管内注射1.0 ml APS，8小时后皮下注射0.5 ml。此后，在接下来的13天里每天皮下注射0.5 ml APS。对照组（18只兔子）未注射APS。在手术前、手术后5分钟、4天、8天以及处死前进行血小板计数。通过拍摄打开的主动脉、将照片投射到硬纸板上、剪出不同病变所占区域并称重硬纸板来估计病变程度。对照组中隆起病变的平均重量比实验组大6至7倍。基于Welsh氏不等方差“t”检验对该差异进行的统计分析具有高度显著性（P小于0.001）。实验组在14天时血小板计数在0至20,000之间。血小板计数小于或等于1,000 mm3的动物中，隆起病变完全得到预防。在第二个实验中，将APS的作用与正常绵羊血清（NSS）进行了比较。APS组中隆起病变同样受到显著抑制。NSS对照组的病变程度与第一个实验的未注射APS组相似。这些发现表明，在富含脂质的隆起病变形成过程中，血栓形成比损伤更重要。
