Muir A, Ruan Q G, Marron M P, She J X
Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville 32610, USA.
Diabetes. 1999 Jan;48(1):219-22. doi: 10.2337/diabetes.48.1.219.
A new viral sequence (IDDMK(1,2)22) similar to the human endogenous retrovirus (HERV)-K10/K18 subfamilies has recently been isolated from the culture supernatants of leukocyte-infiltrated islets from two patients who died at the onset of type 1 diabetes. It was claimed that this endogenous retrovirus is expressed in patients with type 1 diabetes but not in healthy control subjects, suggesting an important role of the retrovirus in beta-cell-specific autoimmunity that results in type 1 diabetes. However, despite exhaustive attempts involving identical and expanded methods of detection, we did not observe the IDDMK(1,2)22 viral sequence in genomic DNA, lymphocyte, or plasma RNA in any subject. Therefore, we believe that the viral sequence is not derived from an endogenous retrovirus and that a role for the retrovirus in the pathogenesis of type 1 diabetes must be reconsidered.
最近，从两名1型糖尿病发病时死亡患者的白细胞浸润胰岛的培养上清液中分离出一种新的病毒序列（IDDMK(1,2)22），它与人类内源性逆转录病毒（HERV）-K10/K18亚家族相似。据称，这种内源性逆转录病毒在1型糖尿病患者中表达，但在健康对照者中不表达，这表明该逆转录病毒在导致1型糖尿病的β细胞特异性自身免疫中起重要作用。然而，尽管我们进行了详尽的尝试，采用相同且扩展的检测方法，但在任何受试者的基因组DNA、淋巴细胞或血浆RNA中均未观察到IDDMK(1,2)22病毒序列。因此，我们认为该病毒序列并非源自内源性逆转录病毒，必须重新考虑该逆转录病毒在1型糖尿病发病机制中的作用。
