Ungar A R, Helde K A, Moon R T
Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington 98195-7750, USA.
Mol Mar Biol Biotechnol. 1998 Dec;7(4):320-6.
We describe a simple method for producing androgenetic haploid embryos in zebrafish. Mature eggs are expressed, and the maternal genome is inactivated by irradiation with short-wave ultraviolet light prior to in vitro fertilization. We demonstrate the absence of maternal genome in the resultant embryos using assays based on morphology and polymerase chain reaction. This technique for the production of androgenetic haploids will be extremely useful for identifying and recovering mutations as a complement to, or in place of, generating gynogenetic haploids.
我们描述了一种在斑马鱼中产生雄核发育单倍体胚胎的简单方法。将成熟卵子取出，在体外受精前用短波紫外线照射使母本基因组失活。我们使用基于形态学和聚合酶链反应的检测方法，证明了所得胚胎中不存在母本基因组。这种产生雄核发育单倍体的技术对于鉴定和恢复突变将极为有用，可作为雌核发育单倍体的补充或替代方法。
