Bohn G, Rücker G, Kröger H
Arch Toxicol. 1976 Jun 8;35(3):213-20. doi: 10.1007/BF00293569.
Three decomposition products of Morazone (ingredient of the pharmaceutical preparation Rosimon-Neu) were observed following heat treatment in acid medium (hydrochloric or tartaric acid). These products were isolated by TLC and identified as bis-antipyryl-methane, phenmetrazine and 4-hydroxymethyl-antipyrine by mass spectrometry and IR-spectroscopy and 1H-nuclear magnetic resonance. Morazone and the metabolite phenmetrazine may be extracted from alkaline urine using chloroform, however acid hydrolysis (pH 1) of the urine before alkaline extraction will improve the sensitivity of detection of morazone by producing the metabolite phenmetrazine in addition to bis-antipyrylmethane. The metabolite 4-hydroxymethyl-antipyrine is barely detectable by TLC from alkaline extraction of urine.
在酸性介质（盐酸或酒石酸）中进行热处理后，观察到了莫拉宗（药物制剂罗西蒙 - 纽的成分）的三种分解产物。通过薄层色谱法分离出这些产物，并通过质谱、红外光谱和氢核磁共振将其鉴定为双安替比林甲烷、苯甲曲秦和4 - 羟甲基安替比林。莫拉宗及其代谢物苯甲曲秦可以用氯仿从碱性尿液中萃取出来，然而在碱性萃取之前对尿液进行酸水解（pH值为1），除了生成双安替比林甲烷外，还会产生代谢物苯甲曲秦，从而提高莫拉宗检测的灵敏度。从碱性尿液萃取物中，通过薄层色谱法几乎检测不到代谢物4 - 羟甲基安替比林。
