Sta Iglesia D D, Vanable J W
Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, D.C., USA.
Wound Repair Regen. 1998 Nov-Dec;6(6):531-42. doi: 10.1046/j.1524-475x.1998.60606.x.
The strength of the electric fields in the vicinity of 1.5 mm circular lesions in the bovine cornea has been found to influence the rate of re-epithelization. A decrease in the field strength by submersion of the lesions or by treating the lesions with the Na+-channel blocker, benzamil, significantly retarded healing. An increase in the field strength of lesions treated with Na+-depleted Hanks' solution, by the addition of direct current, increased epithelization. Epithelization was fastest in wounds with field strengths raised to - 80 mV/mm, more than twice the normal field strength present in wounds maintained in Hanks' solution alone. Epithelization decreased, however, when the field strengths were increased to -120 mV/mm. A similar pattern was also observed when the field's polarity was reversed. By manipulating and monitoring the field strengths, we have been able to show for the first time that increased wound field strengths enhance corneal wound epithelization, and that field strengths with reversed polarity also enhance this epithelization.
已发现牛眼角膜中1.5毫米圆形损伤附近的电场强度会影响重新上皮化的速率。通过将损伤浸入水中或用钠通道阻滞剂苯扎米尔处理损伤来降低电场强度，会显著延缓愈合。用无钠的汉克斯溶液处理损伤后，通过施加直流电增加电场强度，上皮化会加快。在电场强度提高到-80 mV/mm的伤口中，上皮化最快，这比仅在汉克斯溶液中维持的伤口中存在的正常电场强度高出两倍多。然而，当电场强度增加到-120 mV/mm时，上皮化会减少。当电场极性反转时，也观察到类似的模式。通过操纵和监测电场强度，我们首次能够表明，增加伤口电场强度可增强角膜伤口上皮化，并且极性反转的电场强度也可增强这种上皮化。
