Chiang M C, Cragoe E J, Vanable J W
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Dev Biol. 1991 Aug;146(2):377-85. doi: 10.1016/0012-1606(91)90239-y.
The lateral electric fields (LFs) in the vicinity of small wounds made in hindlimb digit tip skin of Notophthalmus viridescens have been measured and manipulated. Healing of these wounds was assessed by crystal violet staining and by histology. Paired experiments were conducted on the animals: the healing of one digit's wound was compared with healing of the contralateral digit's wound whose LF was changed from that of its contralateral wound. When currents were passed through the animal (into one wound and out of the contralateral wound) so that the LF of one wound was zero while the contralateral wound had an enhanced LF, the wounds with the enhanced LF healed more rapidly than the wounds with the zero LF. When digits on one side were treated with 30 microM benzamil in an artificial pond water so that their wound LFs were reduced to approximately zero, and the contralateral wounds were kept in artificial pond water without benzamil so that they had normal wound LFs, there was significantly less epithelization of the benzamil-treated wounds than of the control wounds. This effect on wound healing was reversed by adding currents that restored the normal wound LFs, but not by adding currents that reversed the wound LFs to the opposite polarity. When currents were added to reverse the wound LFs on one side of the animal, leaving the contralateral wounds free of added currents, the wounds with the reversed LFs healed more poorly than the contralateral wounds with normal LFs. These results are consistent with the hypothesis that the intrinsic LFs in the vicinity of wounds promote epithelization of these wounds.
已对绿螈后肢指尖皮肤小伤口附近的侧向电场（LFs）进行了测量和调控。通过结晶紫染色和组织学评估这些伤口的愈合情况。对动物进行了配对实验：将一个趾头伤口的愈合情况与对侧趾头伤口的愈合情况进行比较，对侧趾头伤口的LF与另一侧不同。当电流通过动物（进入一个伤口并从对侧伤口流出），使一个伤口的LF为零而对侧伤口的LF增强时，LF增强的伤口比LF为零的伤口愈合得更快。当一侧的趾头在人工池塘水中用30微摩尔的苯扎明处理，使其伤口LF降低到接近零，而对侧伤口保存在不含苯扎明的人工池塘水中，使其具有正常的伤口LF时，经苯扎明处理的伤口的上皮形成明显少于对照伤口。通过添加能恢复正常伤口LF的电流可逆转这种对伤口愈合的影响，但添加使伤口LF反向为相反极性的电流则不能。当添加电流使动物一侧的伤口LF反向，而对侧伤口不添加电流时，LF反向的伤口比LF正常的对侧伤口愈合得更差。这些结果与伤口附近的固有LF促进这些伤口上皮形成的假说一致。
