Komlos L, Zahavi Z, Dicker D, Luria D, Salman H, Zahavi I
Research Institute for Human Reproduction, Rabin Medical Center, Petach Tiqva, Israel.
Am J Reprod Immunol. 1998 Dec;40(6):418-23. doi: 10.1111/j.1600-0897.1998.tb00428.x.
The possible in vitro immunomodulating effect of beta-estradiol on phytohemagglutinin-stimulated human lymphocyte cultures was studied.
Lymphocyte cultures from 12 healthy men and women aged 25-35 years were set up for 12 hr in the presence and in the absence of beta-estradiol, and the expression of the activation markers CD25, CD69, and CD71 was examined by flow cytometric analysis with specific fluorescent conjugated antibodies.
Although the number of cases is small, in 10 of 12 cases in the presence of beta-estradiol in two different concentrations, a significantly decreased expression of CD69 could be observed. A slight decrease could also be observed for the Interleukin-2 receptor expression; however, the difference, in the presence or absence of beta-estradiol, was not significant.
The results suggest that in vitro addition of beta-estradiol can inhibit, to a certain degree, specific activation markers on phytohemagglutinin-stimulated lymphocytes from young men and women. The present study could not define the role of sex differences because of the small number of samples. A comparison between men and women at various ages in a greater number of cases, as well as studies on activation markers after treatments with estrogens, would be useful.
研究了β-雌二醇对植物血凝素刺激的人淋巴细胞培养物可能的体外免疫调节作用。
在有和没有β-雌二醇的情况下，建立了12名年龄在25 - 35岁的健康男性和女性的淋巴细胞培养物12小时，并使用特异性荧光偶联抗体通过流式细胞术分析检测活化标志物CD25、CD69和CD71的表达。
尽管病例数较少，但在两种不同浓度的β-雌二醇存在下的12例病例中的10例中，可观察到CD69表达显著降低。白细胞介素-2受体表达也可观察到轻微降低；然而，在有或没有β-雌二醇的情况下，差异不显著。
结果表明，体外添加β-雌二醇可在一定程度上抑制年轻男性和女性植物血凝素刺激的淋巴细胞上的特异性活化标志物。由于样本数量少，本研究无法确定性别差异的作用。在更多病例中比较不同年龄的男性和女性，以及研究雌激素治疗后的活化标志物，将是有用的。
