Boland A, Havaux S, Cornelis G R
Microbial Pathogenesis Unit, Christian de Duve Institute of Cellular Pathology and Faculté de Médecine, Brussels, B-1200, Belgium.
Microb Pathog. 1998 Dec;25(6):343-8. doi: 10.1006/mpat.1998.0247.
Virulent Yersinia species (Y. pestis, Y. pseudotuberculosis and Y. enterocolitica) possess a 70 kb virulence plasmid that encodes the Yop virulon. This virulence system allows extracellular bacteria adhering at the surface of eukaryotic cells to secrete and inject bacterial effector proteins, called Yops, into the cytosol of these cells in order to disarm them. These secreted Yop proteins are remarkably conserved among the different species. A Y. enterocolitica O:8 strain was found to secrete a protein antigenically related to YopM but significantly larger. Sequencing of the corresponding gene showed that the protein was a YopM variant with three repeats of one domain. Comparison of the yopM gene of various Yersinia strains by PCR amplification, as well as analysis of the secreted Yop proteins by SDS-PAGE and Western blotting revealed that, unlike the other Yops, the YopM protein shows some heterogeneity.
致病性耶尔森氏菌属（鼠疫耶尔森氏菌、假结核耶尔森氏菌和小肠结肠炎耶尔森氏菌）拥有一个70 kb的毒力质粒，该质粒编码Yop毒力蛋白群。这种毒力系统使附着在真核细胞表面的胞外细菌能够将称为Yop的细菌效应蛋白分泌并注入这些细胞的细胞质中，从而使其失去防御能力。这些分泌的Yop蛋白在不同物种之间具有显著的保守性。发现一株小肠结肠炎耶尔森氏菌O:8菌株分泌一种与YopM抗原相关但明显更大的蛋白质。对相应基因的测序表明，该蛋白质是具有一个结构域三个重复序列的YopM变体。通过PCR扩增对各种耶尔森氏菌菌株的yopM基因进行比较，以及通过SDS-PAGE和蛋白质免疫印迹法对分泌的Yop蛋白进行分析，结果显示，与其他Yop不同，YopM蛋白表现出一定的异质性。
