顶孢霉属真菌对乙烷的氧化作用。
Oxidation of ethane by an Acremonium species.
作者信息
Davies J S, Wellman A M, Zajic J E
出版信息
Appl Environ Microbiol. 1976 Jul;32(1):14-20. doi: 10.1128/aem.32.1.14-20.1976.
Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway of ethane oxidation via ethanol, acetaldehyde, and acetic acid. In vitro studies indicated that ethane-oxidizing activity in such mycelia occurred predominantly in the microsomal fraction of crude homogenates. Microsomal preparations were inactive in the absence of added coenzyme. Marked stimulation of activity was obtained in such preparations with reduced nicotinamide adenine dinucleotide phosphate and to a much lesser degree with nicotinamide adenine dinucleotide phosphate. Ethane oxidation was inhibited by sodium azide and carbon monoxide.
在一株顶孢霉的乙烷培养静止细胞（菌丝体）以及此类菌丝体的无细胞制剂中研究了乙烷氧化。从静止细胞实验中发现了一条通过乙醇、乙醛和乙酸进行乙烷氧化的途径。体外研究表明，此类菌丝体中的乙烷氧化活性主要存在于粗匀浆的微粒体部分。在没有添加辅酶的情况下，微粒体制剂没有活性。用还原型烟酰胺腺嘌呤二核苷酸磷酸可显著刺激此类制剂中的活性，而烟酰胺腺嘌呤二核苷酸磷酸的刺激程度要小得多。叠氮化钠和一氧化碳可抑制乙烷氧化。
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