Chaudry I H, Sayeed M M, Baue A E
Can J Physiol Pharmacol. 1976 Oct;54(5):736-41. doi: 10.1139/y76-102.
Hemorrhagic shock in rats was produced by bleeding the animals to a mean arterial pressure of 40 mmHg, which was maintained for 2 h. Muscles from these animals ('shock' muscles) demonstrated basal glucose uptake values unchanged from control values but, unlike the control muscles, the "shock' muscles showed resistance to the stimulation of glucose uptake by insulin. Infusion of ATP-MgCl2, ADP-MgCl2, adenosine-MgCl2, or GTP-MgCl2 to animals following shock had no effect on basal glucose uptake; however, ATP-MgCl2 but not the other nucleotides permitted insulin to exert its stimulatory effect on such muscles. An optimal insulin effect in ATP-MgCl2 treated 'shock' muscles occurred at an insulin concentration of u.001 U/ml, which is also the concentration required to produce optimal insulin effect in control muscles. Following 1-h incubation in Krebs-HCO3 medium, intracellular ATP contents of 'shock' muscles were about 50% lower than in control muscles. Treatment with ATP-MgCl2 following shock, however, resulted in ATP contents in such muscles similar to those in control muscles. Possible mechanisms for this reversal of insulin resistance by in vivo infusion of ATP-MgCl2 in shock are discussed.
通过将大鼠放血至平均动脉压40 mmHg并维持2小时来制造失血性休克。这些动物的肌肉（“休克”肌肉）显示基础葡萄糖摄取值与对照值无变化，但与对照肌肉不同，“休克”肌肉对胰岛素刺激葡萄糖摄取表现出抵抗。休克后给动物输注ATP-MgCl2、ADP-MgCl2、腺苷-MgCl2或GTP-MgCl2对基础葡萄糖摄取无影响；然而，ATP-MgCl2而非其他核苷酸能使胰岛素对这类肌肉发挥刺激作用。在ATP-MgCl2处理的“休克”肌肉中，胰岛素的最佳效应出现在胰岛素浓度为0.001 U/ml时，这也是在对照肌肉中产生最佳胰岛素效应所需的浓度。在Krebs-HCO3培养基中孵育1小时后，“休克”肌肉的细胞内ATP含量比对照肌肉低约50%。然而，休克后用ATP-MgCl2处理，这类肌肉中的ATP含量与对照肌肉相似。讨论了休克时通过体内输注ATP-MgCl2逆转胰岛素抵抗的可能机制。
