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[水中粪链球菌检测四种方法的比较]

[Comparison of four methods for the detection of fecal streptococci in water].

作者信息

Heiber I, Frahm E, Obst U

机构信息

WFM Wasserforschung Mainz.

出版信息

Zentralbl Hyg Umweltmed. 1998 Dec;201(4-5):357-69.

PMID:9916290
Abstract

The objective of this study was to select an effective and fast method for the detection of socalled fecal streptococci in water by comparing a method according to the German drinking water standard, a membrane filtration method according to the ISO-draft standard 7899/2, the Chromocult Enterococcus Broth (Merck) and the Enterolert-System (IDEXX). The study was based on a collective of 297 water samples derived from different stages of water treatment and distribution, as well as from individual water supplies. The sensitivity, reliability, and selectivity of the single methods in relation to their practicability was evaluated. Concerning false positive and false negative results, the tests were proved by metabolic characterization of the isolated strains. The advantages and disadvantages of the methods resulting from the investigated criteria are discussed. The work is part of a comparative study within the scope of the DIN ad-hoc-committee "fecal streptococci".

摘要

本研究的目的是通过比较德国饮用水标准方法、ISO 标准草案 7899/2 的膜过滤法、Chromocult 肠球菌肉汤(默克公司)和 Enterolert 系统(IDEXX 公司),选择一种有效且快速的检测水中所谓粪链球菌的方法。该研究基于 297 个来自水处理和分配不同阶段以及个别供水的水样集合。评估了单一方法在实用性方面的灵敏度、可靠性和选择性。关于假阳性和假阴性结果,通过对分离菌株的代谢特征进行验证。讨论了根据研究标准得出的方法的优缺点。这项工作是 DIN 特别委员会“粪链球菌”范围内比较研究的一部分。

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