Figueras M J, Inza I, Polo F L, Feliu M T, Guarro J
Unit of Microbiology, Medical School, University Rovira i Virgili, Reus, Spain.
Appl Environ Microbiol. 1996 Jun;62(6):2177-8. doi: 10.1128/aem.62.6.2177-2178.1996.
M-Enterococcus medium, used for the detection and enumeration of fecal streptococci from different types of water, demonstrated a very low specificity. We propose the transfer of membrane filters to bile-esculin medium as a confirmation technique. It has proved to be more reliable, faster to perform, and more effective than the random selection of colonies, which is recommended for confirmation in Standard Methods for the Examination of Water and Wastewater (A.E. Greenberg, L.S. Clesceri, and A.D. Eaton, ed., 1992).
用于检测和计数不同类型水中粪链球菌的M-肠球菌培养基显示出非常低的特异性。我们建议将膜滤器转移至胆汁-七叶苷培养基作为一种确证技术。事实证明,它比随机挑选菌落更可靠、操作更快且更有效,而随机挑选菌落是《水和废水检验标准方法》(A.E. 格林伯格、L.S. 克莱斯切里和A.D. 伊顿编,1992年)中推荐的确证方法。