Drábek J, Vránová K, Ambrůzová Z, Petrek M, Bártová A, Weigl E
Department of Immunology, Teaching Hospital, Olomouc, the Czech Republic.
Bone Marrow Transplant. 1998 Dec;22 Suppl 4:S23-6.
In this article, methodical experience and population data are given for application of PCR-SSP/ARMS method (Phototyping) to HLA class I typing in Olomouc. Phototyping is reliable method suitable for typing of small to medium number of samples. Method is fast enough for use in on-call service, resolution is better than the level of good serology, and price of method is comparable with serology. Our experience and tips are described below. Population data of healthy unrelated individuals for HLA-A, -B, -Cw are given in the tables.
本文给出了在奥洛穆茨将聚合酶链反应-序列特异性引物/扩增阻滞突变系统方法（光定型法）应用于HLA I类分型的系统经验和群体数据。光定型法是一种可靠的方法，适用于中少量样本的分型。该方法速度足够快，可用于随叫随到的服务，分辨率优于良好血清学水平，且方法价格与血清学相当。我们的经验和提示如下。表格中给出了健康无关个体的HLA-A、-B、-Cw群体数据。
