Dressel R, Johnson J P, Günther E
Division of Immunogenetics, University of Göttingen, Germany.
Melanoma Res. 1998 Dec;8(6):482-92. doi: 10.1097/00008390-199812000-00002.
The heat shock response, which is characterized by the induction of heat shock proteins, is known to affect the ability of tumour cells to cope with potentially adverse conditions such as hypoxia, glucose starvation and cytotoxic immune reactions. To assess the heat shock response of melanoma cells, spontaneous and heat shock induced expression of heat shock proteins was analysed in a panel of 17 human melanoma cell lines. Constitutive expression of HSP27, HSP70, HSC70, HSP90alphabeta and GRP94 proteins was found in all the melanoma cell lines, and HSP70 and HSC70 were also induced by heat shock. The major heat inducible HLA-linked HSP70-1 and HSP70-2 genes were analysed at the mRNA level. Basal expression and inducibility varied between the different melanoma cell lines. In addition, in situ hybridization demonstrated heterogeneous expression of these genes among single cells of a given cell line. In general, each melanoma cell line appears to exhibit an individual type of HSP70 expression that might reflect selection during tumour progression and therapy.
热休克反应以热休克蛋白的诱导为特征，已知其会影响肿瘤细胞应对潜在不利条件的能力，如缺氧、葡萄糖饥饿和细胞毒性免疫反应。为评估黑色素瘤细胞的热休克反应，在一组17个人类黑色素瘤细胞系中分析了热休克蛋白的自发表达和热休克诱导表达。在所有黑色素瘤细胞系中均发现了HSP27、HSP70、HSC70、HSP90αβ和GRP94蛋白的组成性表达，并且热休克也可诱导HSP70和HSC70的表达。在mRNA水平分析了主要的热诱导HLA连锁HSP70-1和HSP70-2基因。不同黑色素瘤细胞系之间的基础表达和诱导性有所不同。此外，原位杂交显示在给定细胞系的单个细胞中这些基因的表达存在异质性。总体而言，每个黑色素瘤细胞系似乎都表现出一种独特的HSP70表达类型，这可能反映了肿瘤进展和治疗过程中的选择情况。
