Meyer J M, Stintzi A, Poole K
Laboratoire de Microbiologie et de Génétique, UPRES A 7010, Université Louis Pasteur, Strasbourg, France.
FEMS Microbiol Lett. 1999 Jan 1;170(1):145-50. doi: 10.1111/j.1574-6968.1999.tb13367.x.
FpvA, the ferripyoverdine outer membrane receptor of Pseudomonas aeruginosa ATCC 15692 (PAO1 strain), is not specific to the pyoverdine produced by PAO1, but is also able to recognize the structurally different (ferri)pyoverdine of P. fluorescens ATCC 13525. The specificity of FpvA was assessed by iron uptake competitions using the wild-type strains P. aeruginosa ATCC 15692 and P. fluorescens ATCC 13525 and their respective ferripyoverdines, and by fpvA gene complementation of a FpvA-deficient mutant of P. aeruginosa ATCC 15692. The receptor mutant was able to utilize none of the two pyoverdines, while the same but fpvA-complemented mutant recovered simultaneously the ability to incorporate iron thanks to each of the two siderophores. The broad specificity of recognition of FpvA is viewed as an advantage for the strain in iron competition. Moreover, it allows an interesting approach for the understanding of the recognition mechanism between a (ferri)pyoverdine and its cognate outer membrane receptor.
FpvA是铜绿假单胞菌ATCC 15692（PAO1菌株）的铁载体绿脓菌素外膜受体，它并非特异性地识别PAO1产生的绿脓菌素，还能够识别荧光假单胞菌ATCC 13525结构不同的（铁）绿脓菌素。通过使用野生型菌株铜绿假单胞菌ATCC 15692和荧光假单胞菌ATCC 13525及其各自的铁载体绿脓菌素进行铁摄取竞争实验，以及对铜绿假单胞菌ATCC 15692的FpvA缺陷型突变体进行fpvA基因互补实验，评估了FpvA的特异性。受体突变体无法利用这两种绿脓菌素中的任何一种，而相同但经fpvA互补的突变体由于这两种铁载体中的每一种，同时恢复了摄取铁的能力。FpvA识别的广泛特异性被视为该菌株在铁竞争中的一个优势。此外，它为理解（铁）绿脓菌素与其同源外膜受体之间的识别机制提供了一种有趣的方法。
