Lee Y K, Chang H H, Kim W R, Kim J K, Yoon Y D
Korea Atomic Energy Research Institute, Taejon, Korea.
Arh Hig Rada Toksikol. 1998 Jun;49(2):147-53.
In order to observe the morphological and endocrinological changes of the rat and mouse ovarian follicles by gamma-radiation, rats were whole-body irradiated with doses of 3.2 Gy and 8.0 Gy and mice with 2.9 Gy and 7.2 Gy. Sections of ovaria were examined by light microscopy. Concentrations of progesterone, testosterone, and estradiol in ovarian homogenate were determined by radioimmunoassay techniques. Gamma-radiation resulted in the increased percentage of atretic follicles in the groups killed on day 0, day 4, and day 8 after irradiation. The decrease in granulosa cell viability was found in animals killed on day 4 after irradiation. The finding of the high ratio of testosterone to estradiol compared to that of progesterone to testosterone suggests that aromatase activity--steroid biosynthesis from testosterone to estradiol--in granulosa cell could be affected by gamma-radiation.
为了观察γ射线辐射对大鼠和小鼠卵巢卵泡的形态学和内分泌学变化，对大鼠进行3.2 Gy和8.0 Gy剂量的全身照射，对小鼠进行2.9 Gy和7.2 Gy剂量的全身照射。通过光学显微镜检查卵巢切片。采用放射免疫分析技术测定卵巢匀浆中孕酮、睾酮和雌二醇的浓度。γ射线辐射导致在照射后第0天、第4天和第8天处死的组中闭锁卵泡的百分比增加。在照射后第4天处死的动物中发现颗粒细胞活力下降。与孕酮与睾酮的比例相比，睾酮与雌二醇的比例较高，这一发现表明颗粒细胞中的芳香化酶活性（从睾酮到雌二醇的类固醇生物合成）可能受到γ射线辐射的影响。
