Arnould J P, Hermant A, Levi-Valensi P, Belegaud J
Laboratoire de Toxicologie, Faculté de Pharmacie, Amiens, France.
Pathol Biol (Paris). 1998 Dec;46(10):787-90.
Benzo[a]pyrene is a cigarette smoke component that is metabolized in the human body to the diol-epoxide derivative benzo[a]pyrene-trans- 7,8-dihydrodiol-9,10-epoxide (BPDE-I), which is the final carcinogen. BPDE-I binds covalently to DNA, producing BPDE-I-DNA adducts. A competitive immunoenzymetric assay was used to measure BPDE-I-DNA adducts in blood samples from 58 heavy smokers, 32 men and 26 women, attending a smoking cessation clinic. Cigarette consumption was evaluated based on urinary continine levels. None of the subjects worked in jobs involving exposure to polycyclic aromatic hydrocarbons (e.g., benzo[a]pyrene). Concentrations of BPDE-I-DNA adducts varied with cigarette consumption, ranging from 10.00 to 28.20 fmol/50 micrograms of DNA.
苯并[a]芘是香烟烟雾的一种成分，在人体内代谢为二醇环氧化物衍生物苯并[a]芘 - 反式 - 7,8 - 二氢二醇 - 9,10 - 环氧化物（BPDE - I），这是最终的致癌物。BPDE - I与DNA共价结合，产生BPDE - I - DNA加合物。采用竞争性免疫酶测定法，对一家戒烟诊所的58名重度吸烟者（32名男性和26名女性）的血液样本中的BPDE - I - DNA加合物进行了测量。根据尿中可替宁水平评估香烟消费量。所有受试者均未从事涉及接触多环芳烃（如苯并[a]芘）的工作。BPDE - I - DNA加合物的浓度随香烟消费量而变化，范围为10.00至28.20 fmol/50微克DNA。
