Shamsuddin A K, Sinopoli N T, Hemminki K, Boesch R R, Harris C C
Cancer Res. 1985 Jan;45(1):66-8.
Metabolic activation of benzo(a)pyrene (BP) to its ultimate carcinogenic form, 7 beta, 8 alpha-diol-9 alpha, 10 alpha-benzo(a)pyrene epoxide (BPDE), and the binding of BPDE to DNA are important steps in BP carcinogenicity in experimental animals. Since people of certain occupations are exposed to high concentrations of BP, we have used enzyme-linked immunosorbent assay and ultrasensitive enzymatic radioimmunoassay to measure BPDE:DNA adducts in white blood cells from 2 of these occupational groups. Seven of 28 samples from roofers and 7 of 20 samples from foundry workers were positive for BPDE:DNA adducts (range, 2 to 120 fmol BPDE/50 micrograms DNA). In a group of nine volunteers without these industrial exposures to BP, the two positive DNA samples were from cigarette smokers. Control DNA obtained from human lymphocyte cell line RPMI 4265 was negative. These results indicate that the metabolic activation of BP and formation of BPDE:DNA adducts occurs in humans.
苯并(a)芘（BP）代谢活化成其最终致癌形式——7β,8α-二醇-9α,10α-苯并(a)芘环氧化物（BPDE），以及BPDE与DNA的结合，是BP在实验动物中致癌作用的重要步骤。由于某些职业的人群会接触高浓度的BP，我们使用酶联免疫吸附测定法和超灵敏酶促放射免疫测定法来检测其中两个职业群体白细胞中的BPDE:DNA加合物。来自屋顶工的28个样本中有7个，来自铸造工人的20个样本中有7个的BPDE:DNA加合物呈阳性（范围为2至120 fmol BPDE/50微克DNA）。在一组没有这些BP职业暴露的9名志愿者中，两个DNA阳性样本来自吸烟者。从人淋巴细胞系RPMI 4265获得的对照DNA为阴性。这些结果表明，BP的代谢活化以及BPDE:DNA加合物的形成在人类中会发生。
