垂体腺苷酸环化酶激活多肽1型受体（PAC1-R）在胃嗜铬样细胞上表达：免疫细胞化学和逆转录聚合酶链反应的证据。
The pituitary adenylate cyclase activating polypeptide type 1 receptor (PAC1-R) is expressed on gastric ECL cells: evidence by immunocytochemistry and RT-PCR.
作者信息
Zeng N, Kang T, Lyu R M, Wong H, Wen Y, Walsh J H, Sachs G, Pisegna J R
机构信息
CURE: VA/UCLA Digestive Diseases Research Center, West Los Angeles Veterans Administration Medical Center, USA.
出版信息
Ann N Y Acad Sci. 1998 Dec 11;865:147-56. doi: 10.1111/j.1749-6632.1998.tb11173.x.
Abstract
The current study was undertaken to determine the presence and distribution of PAC1-Rs within the gastric mucosa. Polyclonal antibodies to the carboxyl terminus of the rat PAC1-R were generated and shown to be specific against the PAC1-R expressed in NIH 3T3 cells. Western blot analysis using isolated (approximately 85% pure) ECL cell membranes identified a 48 kD protein consistent with the calculated molecular mass of the cloned PAC1-R. RT/PCR performed using specific primers for the PAC1-R confirmed the presence of splice variants of the rat PAC1-R, but not VPAC1-R or VPAC2-R. These data provide the first direct evidence for the existence of functional PACAP Type I receptors on ECL cells of the gastric mucosa and suggest a potential role for PACAP in the stimulation of gastric acid secretion and in the regulation of the growth of ECL cells.
摘要
本研究旨在确定PAC1受体在胃黏膜中的存在情况及分布。针对大鼠PAC1受体羧基末端产生了多克隆抗体，并证明其对NIH 3T3细胞中表达的PAC1受体具有特异性。使用分离的（约85%纯）肠嗜铬样（ECL）细胞膜进行的蛋白质印迹分析鉴定出一种48 kD的蛋白质，与克隆的PAC1受体的计算分子量一致。使用针对PAC1受体的特异性引物进行的逆转录/聚合酶链反应（RT/PCR）证实了大鼠PAC1受体剪接变体的存在，但未检测到血管活性肠肽受体1（VPAC1-R）或血管活性肠肽受体2（VPAC2-R）。这些数据为胃黏膜ECL细胞上存在功能性I型垂体腺苷酸环化酶激活肽（PACAP）受体提供了首个直接证据，并提示PACAP在刺激胃酸分泌和调节ECL细胞生长中可能发挥作用。
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本文引用的文献
1
PACAP hybrid: a new PACAP receptor antagonist.
Life Sci. 1997;61(6):631-9. doi: 10.1016/s0024-3205(97)00438-4.
2
Selective ligand-induced intracellular calcium changes in a population of rat isolated gastric endocrine cells.
Gastroenterology. 1996 Jun;110(6):1835-46. doi: 10.1053/gast.1996.v110.pm8964409.
3
Cloning and characterization of the signal transduction of four splice variants of the human pituitary adenylate cyclase activating polypeptide receptor. Evidence for dual coupling to adenylate cyclase and phospholipase C.人垂体腺苷酸环化酶激活多肽受体四种剪接变体的信号转导克隆与特性分析。与腺苷酸环化酶和磷脂酶C双重偶联的证据。
J Biol Chem. 1996 Jul 19;271(29):17267-74. doi: 10.1074/jbc.271.29.17267.
4
Differential signal transduction by five splice variants of the PACAP receptor.垂体腺苷酸环化酶激活肽（PACAP）受体的五种剪接变体的差异信号转导
Nature. 1993 Sep 9;365(6442):170-5. doi: 10.1038/365170a0.
5
Molecular cloning and functional expression of the pituitary adenylate cyclase-activating polypeptide type I receptor.垂体腺苷酸环化酶激活多肽I型受体的分子克隆与功能表达
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6345-9. doi: 10.1073/pnas.90.13.6345.
6
Pituitary adenylate cyclase activating polypeptide receptors are present on small cell lung cancer cells.垂体腺苷酸环化酶激活多肽受体存在于小细胞肺癌细胞上。
Peptides. 1993 Mar-Apr;14(2):241-6. doi: 10.1016/0196-9781(93)90036-g.
7
8
The VIP2 receptor: molecular characterisation of a cDNA encoding a novel receptor for vasoactive intestinal peptide.血管活性肠肽2型受体：编码血管活性肠肽新型受体的cDNA的分子特征
FEBS Lett. 1993 Nov 8;334(1):3-8. doi: 10.1016/0014-5793(93)81668-p.
9
Type I receptors for PACAP (a neuropeptide even more important than VIP?).
Biochim Biophys Acta. 1993 Oct 29;1154(2):183-99. doi: 10.1016/0304-4157(93)90011-c.
10
Histamine as an intermediate growth factor in genesis of gastric ECLomas associated with hypergastrinemia in mastomys.组胺作为与多乳鼠高胃泌素血症相关的胃嗜铬样细胞瘤发生过程中的一种中间生长因子。
Dig Dis Sci. 1994 Jul;39(7):1446-53. doi: 10.1007/BF02088047.
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